Anti-TRAF7 Rabbit Polyclonal Antibody for Western Blot and ELISA - Q6Q0C0

Core Product Specifications and Parameters

Biological Background: TRAF7 Function and Localization

• TRAF7 is an E3 ubiquitin and SUMO-protein ligase that plays a role in innate immunity, inflammation, and apoptosis. Related references: PMID:15001576, PMID:37086853
• It potentiates MAP3K3-mediated activation of JUN/AP1 and DDIT3 transcriptional regulators. Related references: PMID:14743216
• TRAF7 negatively regulates RLR-mediated innate immunity by promoting 'Lys-48'-linked ubiquitination of TBK1 through its RING domain. Related references: PMID:37086853
• It promotes 'Lys-29'-linked polyubiquitination of NEMO/IKBKG and RELA, targeting them for lysosomal degradation and reducing NF-kappa-B transcriptional activity. Related references: PMID:21518757
• TRAF7 localizes to cytoplasmic vesicles, the cytoplasm, and the nucleus, consistent with its regulatory roles in signaling pathways.
• The protein contains a RING finger domain and WD repeats, and is classified as a zinc-finger protein and transferase.
• TRAF7 is ubiquitously expressed, with highest levels in skeletal muscle, heart, colon, spleen, kidney, liver, and placenta.
• Alternative splicing produces multiple isoforms, and the protein may be regulated by phosphorylation.

Experimental Guidance and Technical Tips

• For western blot, consider using a whole-cell lysate from human or rat cell lines known to express TRAF7, and validate band specificity around 75 kDa.
• Optimize ELISA conditions by titrating the antibody concentration and using recombinant TRAF7 protein as a standard.
• Since TRAF7 has multiple isoforms, confirm detection of the intended target by molecular weight and, if possible, by isoform-specific controls.
• The immunogen spans amino acids 260–400, a central region that may recognize multiple isoforms; consider cross-reactivity experiments to verify specificity.
• For studying TRAF7's role in immune signaling, combine this antibody with pathway-specific inhibitors or activators in functional assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop partner for IVD raw-material sourcing, providing validated antibody pairs, optimized polyclonal antibodies like this anti-TRAF7 antibody, and bulk ancillary reagents for immunology and inflammation research. We streamline procurement from concept to clinic, ensuring reliable access to rare target raw materials and comprehensive technical support for your diagnostic development needs.