Anti-TRAF7 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q6Q0C0

Core Product Specifications and Parameters

Biological Background: TRAF7 Function and Localization

• TRAF7 (TNF receptor-associated factor 7) is an E3 ubiquitin and SUMO-protein ligase that functions in innate immunity, inflammation, and apoptosis. Related references: PMID:15001576 PMID:37086853
• It potentiates MAP3K3-mediated activation of JUN/AP1 and DDIT3 transcriptional regulators, and modulates MAPK1/3 phosphorylation via MAP3K3 interaction. Related references: PMID:14743216
• TRAF7 negatively regulates RLR-mediated innate immunity by promoting Lys-48-linked ubiquitination of TBK1 through its RING domain, thereby inhibiting antiviral response. Related references: PMID:37086853
• It directs Lys-29-linked polyubiquitination of NEMO (IKBKG) and RELA, targeting these proteins for lysosomal degradation and downregulating NF-kappa-B transcriptional activity. Related references: PMID:21518757
• TRAF7 negatively regulates MYB transcriptional activity by SUMOylation-mediated cytosolic sequestration (By similarity).
• Subcellularly, TRAF7 localizes to cytoplasmic vesicles, cytoplasm, and nucleus.
• It is ubiquitously expressed, with highest levels in skeletal muscle, heart, colon, spleen, kidney, liver, and placenta.
• The protein contains a RING finger domain, WD repeats, zinc-finger motifs, and is subject to phosphorylation. It is associated with disease variants and plays roles in transcription regulation, ubiquitin conjugation, and apoptosis.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band at approximately 75 kDa corresponding to endogenous TRAF7. Titrate the antibody to achieve optimal signal-to-noise ratio.
• In immunohistochemistry (IHC-P) and immunofluorescence/immunocytochemistry (IF/ICC), validate staining specificity using relevant positive and negative control tissues/cells.
• The immunogen corresponds to amino acids 380-479, a region that may be conserved across human, mouse, and rat; however, cross-reactivity should be confirmed experimentally.
• As a polyclonal antibody, consider potential lot-to-lot variability; re-optimize dilution for each new lot.
• For ELISA applications, antigen coating and detection conditions may need optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a one-stop sourcing solution for IVD raw materials, covering targets like TRAF7 involved in ubiquitin ligase pathways and innate immunity. Beyond this high-quality polyclonal antibody, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized raw-material sourcing for rare or difficult targets to accelerate your assay development.