Anti-TRAF6BP/TAX1BP1 Polyclonal Antibody for IHC-P and ELISA - Q86VP1

Core Product Specifications and Parameters

Biological Background: TRAF6BP/TAX1BP1 Function and Localization

• Tax1-binding protein 1 (TAX1BP1, also known as TRAF6-binding protein, T6BP, TXBP151, CALCOCO3) is a ubiquitin-binding adapter protein that participates in inflammatory, antiviral, innate immune processes, and selective autophagy.
• Functions as a key negative regulator of NF-kB and IRF3 signaling pathways by acting as an adapter for the ubiquitin-editing enzyme A20/TNFAIP3 to bind and inactivate its substrates, such as TRAF6 and RIPK1, thereby disrupting IL-1 and TNF-induced NF-kB signaling. Related references: PMID:17703191
• Disrupts the interactions between the E3 ubiquitin ligase TRAF3 and TBK1/IKBKE to attenuate 'Lys63'-linked polyubiquitination of TBK1 and subsequent IFN-beta production. Related references: PMID:21885437
• Acts as a macroautophagy/autophagy receptor facilitating the xenophagic clearance of pathogenic bacteria like Salmonella typhimurium and Mycobacterium tuberculosis, and recruits RB1CC1 to SQSTM1-ubiquitin condensates to promote their autophagic degradation. Related references: PMID:26451915 PMID:33226137 PMID:34471133
• Mediates the autophagic degradation of other substrates, including TICAM1, and inhibits virus-induced apoptosis by inducing 'Lys-48'-linked polyubiquitination and degradation of MAVS via recruitment of the E3 ligase ITCH. Related references: PMID:28898289 PMID:27736772
• Subcellular localization includes cytoplasm, mitochondria, preautophagosomal structures, cytoplasmic vesicles, and autophagosomes.
• Ubiquitously expressed in all tissues tested.
• Contains a zinc-finger domain and coiled-coil regions; subject to phosphorylation and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 709-789), which may influence epitope accessibility; consider validating the antibody in the relevant sample system.
• For IHC-P, optimize antigen retrieval conditions and validate with known positive and negative human tissue controls. Rat cross-reactivity offers potential for translational models.
• For ELISA, the polyclonal nature may provide sensitive detection; pairing with a monoclonal antibody could be evaluated for sandwich assay development.
• Verify the observed molecular weight (~91kDa) in your experimental system, as post-translational modifications or isoforms may shift migration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to IVD raw materials, technical services, and expert consulting—covering every stage from concept to clinic. This anti-TRAF6BP/TAX1BP1 polyclonal antibody supports research in NF-kB signaling, autophagy, and innate immunity, areas critical for inflammatory disease and infectious disease diagnostics. Through our global network, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and efficient sourcing for rare-target raw materials, empowering your assay development and manufacturing workflows.