Anti-TRAF6 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y4K3

Core Product Specifications and Parameters

Biological Background: TRAF6 Function and Localization

• TRAF6 (TNF receptor-associated factor 6) is a member of the TRAF family of adapter proteins and E3 ubiquitin ligases.
• It catalyzes the synthesis of Lys-63-linked polyubiquitin chains on target proteins including ECSIT, IKBKG, IRAK1, AKT1, and AKT2.
  Related references: PMID:11057907, PMID:18347055, PMID:19465916
• This ubiquitination activity leads to the activation of the NF-kB and JUN signaling pathways.
  Related references: PMID:16378096, PMID:17135271, PMID:17703191
• TRAF6 represses c-Myb-mediated transactivation in B-lymphocytes.
  Related references: PMID:18093978, PMID:18758450
• It functions as an adapter in signal transduction initiated via TNF receptor, IL-1 receptor, and IL-17 receptor.
  Related references: PMID:12140561, PMID:19825828, PMID:8837778
• The protein localizes to the cytoplasm, cell cortex, nucleus, and lipid droplets, and is broadly expressed in tissues including heart, brain, and skeletal muscle.
• TRAF6 contains a RING finger domain, multiple zinc fingers, and a coiled-coil region, and is implicated in osteogenesis and immune regulation.

Experimental Guidance and Technical Tips

• For Western blot (WB), the expected band is approximately 60 kDa; consider loading appropriate positive controls and validating in physiologically relevant cell or tissue lysates.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization protocols to detect TRAF6 in its multiple subcellular locations (cytoplasm, nucleus, lipid droplets).
• The antibody is reported to cross-react with mouse and rat TRAF6; confirm sequence homology and validate species-specific reactivity in pilot experiments before large-scale use.
• As the immunogen is a synthetic peptide, reactivity may be limited to the immunizing region; consider testing in the context of native and denatured protein conformations across intended applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibodies, bulk reagents, and technical support from concept to clinic. For researchers studying TRAF6 and NF-κB pathways, we provide a reliable supply of polyclonal and monoclonal antibodies, antigen materials, and ancillary reagents tailored to diagnostic development and immunotherapy research.