Anti-TRAF6 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q9Y4K3

Core Product Specifications and Parameters

Biological Background: TRAF6 Function and Localization

• TRAF6 (TNF receptor-associated factor 6) is an E3 ubiquitin-protein ligase and signal transducer, also known as RING finger protein 85 (RNF85) and Interleukin-1 signal transducer.
• It mediates the synthesis of 'Lys-63'-linked polyubiquitin chains, leading to activation of NF-kappa-B and JUN signaling pathways (PubMed:16378096, PubMed:17135271, PubMed:17703191). It also ubiquitinates unanchored polyubiquitin chains to activate MAP3K7 (PubMed:19675569). Related references: PMID:16378096, PMID:17135271, PMID:17703191, PMID:19675569
• As an adapter protein, TRAF6 plays a role in signal transduction initiated via TNF receptor, IL-1 receptor, and IL-17 receptor (PubMed:12140561, PubMed:19825828, PubMed:8837778). Related references: PMID:12140561, PMID:19825828, PMID:8837778
• TRAF6 represses c-Myb-mediated transactivation in B-lymphocytes (PubMed:18093978, PubMed:18758450). Related references: PMID:18093978, PMID:18758450
• In T-cell receptor (TCR) signaling, TRAF6 ubiquitinates the linker for activation of T cells (LAT), contributing to downstream signaling (PubMed:23514740, PubMed:25907557). Related references: PMID:23514740, PMID:25907557
• TRAF6 localizes to the cytoplasm, cell cortex, nucleus, and lipid droplets, indicating diverse subcellular functions.
• It is broadly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-274) of human TRAF6, which includes the RING finger domain and zinc-finger motifs; consider epitope accessibility when designing immunoassays.
• For Western blotting, TRAF6 has a predicted molecular weight of ~60 kDa; validate with appropriate molecular weight markers and account for possible post-translational modifications.
• This antibody is reactive with human, mouse, and rat TRAF6; confirm species-specific reactivity in your experimental system before quantitative studies.
• For immunoprecipitation, test antibody performance under both native and denaturing conditions, as appropriate for your target-protein interactions.
• In ELISA applications, optimize antibody concentration and consider using a matched antibody pair for enhanced sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material supplier offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-TRAF6 antibody supports immunology and cell signaling research, and our team can assist with customized procurement and technical consulting. Partner with CamelBio to accelerate your diagnostic development from concept to clinic.