Anti-TRAF6 Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q9Y4K3

Core Product Specifications and Parameters

Biological Background: TRAF6 Function and Localization

• TRAF6 is an E3 ubiquitin ligase that catalyzes Lys-63-linked polyubiquitination of substrates like ECSIT, IKBKG, IRAK1, and AKT1/2, and also mediates ubiquitination of free polyubiquitin chains to activate MAP3K7, ultimately leading to NF-kappa-B and JUN activation. Related references: PMID:11057907 PMID:18347055 PMID:19465916 PMID:19675569 PMID:16378096 PMID:17135271 PMID:17703191
• It serves as an adapter protein in signaling cascades initiated by TNF receptor, IL-1 receptor, and IL-17 receptor. Related references: PMID:12140561 PMID:8837778 PMID:19825828
• In B-lymphocytes, TRAF6 represses c-Myb-mediated transactivation, influencing transcriptional regulation. Related references: PMID:18093978 PMID:18758450
• It catalyzes assembly of heterotypic Lys-63/Lys-48 branched ubiquitin chains that protect Lys-63 linkages from CYLD deubiquitination, ensuring persistent signaling. Related references: PMID:27746020
• TRAF6 participates in T cell receptor (TCR) signaling by ubiquitinating the adaptor protein LAT. Related references: PMID:23514740 PMID:25907557
• It also plays roles in dendritic cell maturation, osteoclast differentiation, and CD40-mediated ERK activation (By similarity).
• Subcellular localization includes cytoplasm, cell cortex, nucleus, and lipid droplets, reflecting its diverse functions.
• Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Contains a RING-type zinc finger and coiled coil domain; associated with DNA damage and immune response.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 100-200) spans a region that includes part of the RING-type zinc finger domain, important for E3 ligase activity. When performing Western blot, a band at approximately 60 kDa is anticipated; validate with positive and negative controls.
• For immunohistochemistry (IHC-P), consider using heart or brain tissue sections as positive controls, given TRAF6's ubiquitous expression.
• In immunoprecipitation and ELISA applications, optimize antibody dilution and buffer conditions to ensure specific binding.
• Cross-reactivity with mouse and rat makes this antibody suitable for studies across species; nevertheless, verify reactivity in your relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material supplier, offering validated antibodies, bulk ancillary reagents, and rare-target procurement to diagnostic manufacturers, laboratories, and research institutes. This anti-TRAF6 rabbit monoclonal antibody exemplifies our commitment to supporting immunoassay development and signaling research, providing reliable tools for NF-kappa-B, JNK, and immune pathway studies. We serve as a one-stop bridge for all your raw material needs, from concept to clinic.