Anti-TRAF3IP2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O43734

Core Product Specifications and Parameters

Biological Background: TRAF3IP2 Function and Localization

• TRAF3IP2 (also known as ACT1, CIKS) is an E3 ubiquitin ligase that catalyzes Lys-63-linked polyubiquitination, enhancing protein-protein interactions and downstream cell signaling. Related references: PMID:19825828
• It serves as an essential adaptor in IL-17A-mediated inflammatory signaling, interacting with IL-17RA and IL-17RC receptor chains through SEFIR domains and catalyzing Lys-63-linked polyubiquitination of TRAF6, which leads to NF-κB and MAPK pathway activation. Related references: PMID:19825828 PMID:24120361
• TRAF3IP2 is widely expressed across human tissues.
• The gene undergoes alternative splicing, producing multiple isoforms, and disease-associated variants are linked to inflammatory disorders such as CANDLE syndrome and psoriasis susceptibility (PSORS13).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–300) of human TRAF3IP2; consider this when assessing epitope accessibility in different assay formats.
• For Western blot, a band of approximately 65 kDa is expected in human samples; validate the observed molecular weight in your system, as post-translational modifications or isoform variations may cause shifts.
• Cross-reactivity has been observed with mouse and rat TRAF3IP2; confirm specificity and sensitivity in the target species using appropriate controls.
• In immunofluorescence/ICC, optimize fixation and permeabilization conditions, as the subcellular localization may influence antigen detection.
• For ELISA applications, consider using the antibody as a capture or detection reagent after verifying performance with your specific antigen and matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. We specialize in delivering reliable raw materials for targets like TRAF3IP2, which plays a pivotal role in IL-17 signaling and autoimmune disease research. From sourcing rare targets to supporting assay development, CamelBio accelerates your path from concept to clinic.