Anti-TRAF3 Rabbit Polyclonal Antibody for WB - Q13114

Core Product Specifications and Parameters

Biological Background: TRAF3 Function and Localization

• TRAF3 (TNF receptor-associated factor 3) is encoded by the TRAF3 gene (GeneID: 7187) and belongs to the TNF receptor-associated factor family. Alternative names include CRAF1, CD40BP, LAP1, and RING-type E3 ubiquitin transferase TRAF3.
• It functions as a cytoplasmic E3 ubiquitin ligase that regulates multiple signaling pathways, including NF-κB, MAPK, and IRF, controlling immune and inflammatory responses. Related references: PMID:33148796, PMID:33608556
• TRAF3 promotes Lys-63-linked polyubiquitination of substrates like ASC in TLR/RLR signaling, activating type I interferon and inflammasome pathways. Related references: PMID:25847972, PMID:27980081
• It acts as a negative regulator of the alternative NF-κB pathway, inhibiting NFKB2 processing and promoting MAP3K14 degradation, crucial for B-cell survival and lymphoid organ development.
• Upon TLR4-mediated bacterial expulsion, TRAF3 undergoes Lys-33-linked polyubiquitination and recruits RALGDS to trigger exocyst complex-dependent bacterial clearance. Related references: PMID:27438768
• Subcellularly, TRAF3 localizes to the cytoplasm, endosomes, and mitochondria, reflecting its diverse signaling roles.
• Post-translational modifications include phosphorylation and ubiquitination (Lys-48, Lys-63, Lys-33); it features zinc-finger domains and coiled-coil regions.
• The protein has a molecular weight of ~64 kDa (calculated 64490 Da) and is subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-100 amino acids of human TRAF3; antibody performance may be optimal when the epitope is accessible in native or denatured conformations.
• For Western blotting, expect a band at ~64 kDa in human, mouse, and rat samples. Consider reducing conditions to resolve monomeric TRAF3.
• For IHC-P and IF/ICC, validate signal specificity using knockout or knockdown controls if available.
• ELISA performance may be influenced by coating antigen and detection format; pilot experiments with standard curves are recommended.

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