Anti-TRAF3 Polyclonal Antibody for WB and ELISA - Q13114

Core Product Specifications and Parameters

Biological Background: TRAF3 Function and Localization

• TRAF3 is a cytoplasmic E3 ubiquitin ligase that modulates NF-κB, MAPK, and IRF pathways, crucial in immune and inflammatory responses.
  Related references: PMID:33148796 PMID:33608556
• In innate immunity, TRAF3 catalyzes 'Lys-63'-linked polyubiquitination of substrates such as ASC to initiate type I interferon production and inflammasome activation.
  Related references: PMID:25847972 PMID:27980081
• Upon TLR4 stimulation, TRAF3 negatively regulates NF-κB and its 'Lys-48'-linked degradation is required for MAPK activation and cytokine release; 'Lys-33'-linked ubiquitination diverts it to bacterial expulsion.
  Related references: PMID:27438768
• TRAF3 constitutively suppresses the alternative NF-κB pathway by targeting MAP3K14 for proteasomal degradation, thereby controlling B cell survival, lymphoid organogenesis, and antibody class switching.
• It localizes to the cytoplasm, endosomes, and mitochondria, consistent with its multifaceted signaling roles.
• The protein undergoes post-translational modifications, including phosphorylation and ubiquitination (Lys-33, Lys-48, Lys-63), and is implicated in host-virus interactions and apoptosis.

Experimental Guidance and Technical Tips

• For Western blotting, a predominant band at approximately 64 kDa is expected; verify specificity using TRAF3‑knockout cell lysates or siRNA‑mediated knockdown controls.
• Since the immunogen spans the N‑terminal region (amino acids 1–100), confirm recognition of mouse and rat orthologs by sequence alignment and preliminary cross‑species testing.
• For ELISA applications, optimize antigen coating concentration and antibody dilution empirically; the antibody may be suitable for both direct and indirect detection formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering a broad portfolio of validated antibodies and ancillary reagents. Our TRAF3 polyclonal antibody complements our expertise in innate immunity and inflammatory disease targets, and we can supply matched pairs, bulk orders, and custom sourcing of rare reagents. Let us streamline your procurement so you can focus on advancing your diagnostic pipeline.