Anti-TRAF2 Rabbit Polyclonal Antibody for WB and ELISA - Q12933

Core Product Specifications and Parameters

Biological Background: TRAF2 Function and Localization

• TRAF2 (TNF receptor-associated factor 2) is an E3 ubiquitin-protein ligase that functions as a central regulator of cell survival and apoptosis by mediating activation of NF-κB and JNK signaling pathways. Related references: PMID:10346818 PMID:11784851 PMID:12917689
• It catalyzes Lys-63-linked ubiquitination of various target proteins, including BIRC3, IKBKE, MLST8, RIPK1, and TICAM1, thereby modulating immune and stress responses. Related references: PMID:23453969 PMID:28489822
• TRAF2 is an essential component of multiple E3 ubiquitin ligase complexes, facilitating the ubiquitination of substrates and regulating mTORC1/mTORC2 assembly through MLST8 ubiquitination. Related references: PMID:15383523 PMID:18981220 PMID:28489822
• The protein is primarily localized to the cytoplasm and contains characteristic domains such as a RING-type zinc finger, coiled-coil region, and TRAF domain, enabling its diverse protein interactions and ubiquitin ligase activity.
• TRAF2 undergoes post-translational modifications including acetylation and phosphorylation, which may influence its activity and interactions in signaling pathways.
• Alternative splicing of the TRAF2 gene produces multiple isoforms, potentially contributing to functional diversity in different cellular contexts.
• Required for normal antibody isotype switching from IgM to IgG, highlighting its role in adaptive immune responses (By similarity).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the TRAF domain, which is highly conserved across human, mouse, and rat, supporting the antibody's cross-reactivity; validate in the relevant sample system.
• For Western blot, consider titrating the antibody to determine the optimal working dilution and include appropriate loading controls and molecular weight markers.
• To confirm specificity, consider using lysates from cells overexpressing or with siRNA-mediated knockdown of TRAF2, as the antibody is polyclonal.
• For ELISA applications, optimize coating antigen concentration and antibody dilution using standard protocols; ensure the secondary antibody is compatible with host species (rabbit).
• Upon receipt, aliquot and store at -20°C or as recommended to maintain stability; avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to high-quality IVD raw materials, from discovery to clinical application. For targets like TRAF2, involved in apoptosis and immune signaling, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents in bulk. Our supply network also supports the sourcing of rare and custom targets, ensuring a streamlined procurement experience for your entire workflow.