Anti-TRAF2 Polyclonal Antibody for WB, IHC-P, ELISA - Q12933

Core Product Specifications and Parameters

Biological Background: TRAF2 Function and Localization

• TRAF2 (TNF receptor-associated factor 2), also known as TRAP3, is an E3 ubiquitin-protein ligase that plays a central role in the regulation of cell survival and apoptosis by mediating activation of NF-kappa-B and JNK. Related references: PMID:10346818, PMID:11784851, PMID:12917689
• It catalyzes 'Lys-63'-linked ubiquitination of target proteins such as BIRC3, IKBKE, MLST8, RIPK1, and TICAM1, influencing diverse signaling cascades. Related references: PMID:23453969, PMID:28489822
• As an essential constituent of several E3 ubiquitin-protein ligase complexes, TRAF2 facilitates the ubiquitination of target proteins by bringing them into contact with other E3 ligases. Related references: PMID:15383523, PMID:18981220
• TRAF2 regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation, a function independent of its RING-type zinc finger domain. Related references: PMID:11907583, PMID:19506082
• It promotes 'Lys-63'-linked ubiquitination of MLST8 to regulate mTORC1 and mTORC2 assembly, inhibiting mTORC2 formation while facilitating mTORC1 assembly. Related references: PMID:28489822
• TRAF2 mediates activation of NF-kappa-B by EIF2AK2/PKR, and is required for normal antibody isotype switching from IgM to IgG. Related references: PMID:15121867
• Localized to the cytoplasm, TRAF2 contains characteristic domains including a RING-type zinc finger and a coiled coil region, and is subject to phosphorylation and acetylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-300) of human TRAF2, which encompasses the RING domain. This region is highly conserved, consistent with the antibody's cross-reactivity with mouse and rat.
• For Western blotting, a band around 56 kDa is expected. Multiple bands may appear due to post-translational modifications or alternative splicing isoforms; consider validating with appropriate controls.
• For immunohistochemistry (IHC-P), standard antigen retrieval procedures are recommended. Optimize antibody dilution and incubation conditions using positive and negative control tissues.
• For ELISA, titrate the antibody to determine the optimal working concentration for your specific assay format and sample type.
• Since TRAF2 is involved in multiple signaling complexes, co-immunoprecipitation or proximity ligation assays could be explored to study interaction partners, but these applications have not been validated with this product and require independent optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with convenient one-stop access to essential IVD raw materials, including the TRAF2 Rabbit pAb for signal transduction and apoptosis studies. We offer validated antibody pairs, bulk monoclonal and polyclonal antibodies, auxiliary reagents, and specialized sourcing for rare targets to streamline your assay development from concept to clinic. Whatever your workflow demands, rely on CamelBio to bridge your raw-material needs with comprehensive supply capabilities.