Anti-TRAF2 Monoclonal Antibody (KD Validated) for WB, IP, ELISA - Q12933

Core Product Specifications and Parameters

Biological Background: TRAF2 Function and Localization

• TRAF2 (TNF receptor-associated factor 2), also known as TRAP, TRAP3, and RNF117, is an E3 ubiquitin-protein ligase that regulates activation of NF-kappa-B and JNK, playing a central role in cell survival and apoptosis (PubMed:10346818, PubMed:11784851, PubMed:12917689). Related references: PMID:10346818 PMID:11784851 PMID:12917689
• It catalyzes 'Lys-63'-linked ubiquitination of target proteins such as BIRC3, IKBKE, MLST8, RIPK1, and TICAM1, essential for signal transduction (PubMed:23453969, PubMed:28489822). Related references: PMID:23453969 PMID:28489822
• TRAF2 is an essential constituent of several E3 ubiquitin ligase complexes, promoting ubiquitination by bridging substrates with other E3 ligases (PubMed:15383523, PubMed:18981220). Related references: PMID:15383523 PMID:18981220
• It regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and degradation, independent of its RING domain (PubMed:11907583, PubMed:19506082). Related references: PMID:11907583 PMID:19506082
• TRAF2 mediates activation of NF-kappa-B by EIF2AK2/PKR, linking innate immunity and stress responses (PubMed:15121867). Related references: PMID:15121867
• It acts as a regulator of mTORC1 and mTORC2 assembly by mediating 'Lys-63'-linked ubiquitination of MLST8, inhibiting mTORC2 while facilitating mTORC1 (PubMed:28489822). Related references: PMID:28489822
• Subcellular localization: predominantly cytoplasmic.
• Keywords associated with TRAF2 include zinc-finger (RING-type), coiled coil, alternative splicing, acetylation, phosphorylation, and Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 190–350 of human TRAF2) spans a conserved region, consistent with reported cross-reactivity to mouse and rat.
• In Western blot (WB), expect multiple bands between 53–61 kDa due to alternative splicing and post-translational modifications; always validate using a relevant positive control lysate.
• For immunoprecipitation (IP), consider using a crosslinker or appropriate isotype control to minimize antibody chain interference.
• When performing ELISA, confirm specificity with recombinant TRAF2 or knockdown/knockout cell lysates and pair with a validated detection antibody.
• Optimize antibody concentration for your specific sample type and assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated monoclonal antibodies such as this anti-TRAF2 clone—an essential tool for dissecting NF-κB signaling, apoptosis, and immune regulation. Beyond single reagents, our integrated supply capabilities cover bulk ancillary reagents, customized antibody development, and rare target procurement, accelerating your assay development from concept to clinic.