Anti-TRADD Rabbit Polyclonal Antibody for WB & ELISA - Q15628

Core Product Specifications and Parameters

Biological Background: TRADD Function and Localization

• TRADD (TNFRSF1A-associated via death domain) is a 34 kDa adapter protein encoded by the TRADD gene, also known as 9130005N23Rik.
• It functions as a crucial adaptor molecule for TNFRSF1A/TNFR1, specifically binding to the cytoplasmic domain of activated TNFR1 to mediate interaction with FADD, thereby linking receptor activation to downstream signaling cascades. Related references: PMID:23955153 PMID:7758105 PMID:8612133
• Overexpression of TRADD induces two major TNF-mediated responses: apoptosis and activation of the NF-κB transcription factor, highlighting its central role in inflammatory and cell death pathways. Related references: PMID:7758105 PMID:8612133
• The nuclear form of TRADD acts as a tumor suppressor by disrupting the interaction between the E3 ubiquitin ligase TRIP12 and the p19ARF isoform of CDKN2A, thereby preventing ubiquitination and degradation of ARF.
• TRADD is localized in the nucleus, cytoplasm, and cytoskeleton, reflecting its dynamic shuttling and diverse functional roles.
• It is ubiquitously expressed across all examined tissues, underscoring its fundamental role in TNF signaling.
• Keywords associated with TRADD include: 3D-structure, alternative splicing, apoptosis, glycoprotein, and reference proteome, indicating its structural characterization and involvement in programmed cell death.

Experimental Guidance and Technical Tips

• Anti-TRADD rabbit polyclonal antibody is raised against a recombinant protein encoding amino acids 199–312 of human TRADD; consider using this immunogen design to evaluate specificity against fragments or isoforms.
• For Western blot (WB), TRADD has a predicted molecular weight of ~34 kDa; validate detection in positive control lysates (e.g., HeLa, Jurkat) known to express TRADD.
• For ELISA applications, assess appropriate coating and detection conditions, potentially pairing with a matched detection antibody if developing a sandwich immunoassay.
• Since the antibody shows cross-reactivity with human and mouse TRADD, verify performance in both species' samples to confirm consistent recognition.
• Polyclonal antibodies may exhibit lot-to-lot variability; validate each new batch in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials, including validated antibodies, antigens, and ancillary reagents. For research on TNF signaling, apoptosis, and NF-κB pathways, we offer a comprehensive portfolio of anti-TRADD antibodies and related proteins to support your assay development needs. Our one-stop sourcing model streamlines procurement, from individual reagents to bulk custom orders, ensuring continuity and reliability for your diagnostic workflows.