Anti-TRAC Rabbit Polyclonal Antibody for WB and ELISA - P01848

Core Product Specifications and Parameters

Biological Background: TRAC Function and Localization

• TRAC encodes the constant region of the T cell receptor (TCR) alpha chain, which pairs with the TCR beta chain to form the αβ TCR complex on the surface of T lymphocytes.
• Recognition of peptide-major histocompatibility complex (pMHC) antigens on antigen-presenting cells by αβ TCRs is crucial for adaptive immunity. Related references: PMID:25493333
• Upon pMHC binding, the TCR-CD3 complex triggers intracellular signaling: LCK phosphorylates CD3 ITAMs, leading to ZAP70 recruitment. Related references: PMID:24600447
• ZAP70 phosphorylates LAT, assembling the LAT signalosome and activating downstream calcium, MAPK, and NF-κB pathways that drive T cell activation and differentiation. Related references: PMID:23524462
• TCR alpha chain diversity arises from V-J recombination in the thymus; intrathymic selection ensures a self-MHC restricted, non-autoaggressive repertoire. Post-thymic pMHC interactions further shape TCR structural and functional avidity. Related references: PMID:15040585
• TRAC is a single-pass type I membrane protein with a transmembrane helix and a short cytoplasmic tail; it localizes to the plasma membrane.
• The constant domain contains conserved cysteines forming an intrachain disulfide bond and is N-glycosylated, contributing to structural integrity.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular constant domain (aa 20–176), enabling recognition of native and denatured TCR alpha chains across variable regions.
• For Western blot, consider reducing conditions to observe the ~16 kDa band; glycosylation may cause minor size shifts.
• In ELISA applications, this polyclonal antibody may serve as a capture or detection reagent after thorough assay format validation.
• Cross-reactivity with mouse TRAC has been reported; validate compatibility in your specific mouse strain and tissue type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a reliable sourcing partner, we offer this anti-TRAC polyclonal antibody alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized rare-target procurement to accelerate your T cell immunology research and assay development.