Anti-TRA2B Rabbit Monoclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - P62995

Core Product Specifications and Parameters

Biological Background: TRA2B Function and Localization

• TRA2B (Transformer-2 protein homolog beta), also known as SFRS10, is a sequence-specific RNA-binding protein that regulates pre-mRNA splicing, either activating or suppressing exon inclusion depending on the target transcript.
• It functionally interacts with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2 gene, and also activates MAPT/Tau exon 10 splicing, linking it to neurodegenerative pathways.
• The protein belongs to the serine/arginine-rich (SR) family of splicing factors, characterized by an RNA recognition motif (RRM) and C-terminal RS domain rich in arginine and serine residues.
• TRA2B is predominantly localized to the nucleus, consistent with its role in pre-mRNA processing.
• It shows highest expression in heart, skeletal muscle, and pancreas, with lower levels in kidney, placenta, and brain.
• Post-translational modifications include phosphorylation, acetylation, methylation, and ubiquitination, which likely modulate its splicing activity and protein interactions.
• Clinically, TRA2B is associated with epilepsy and intellectual disability, as indicated by keyword annotations, highlighting its importance in neuronal function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-100 of human TRA2B (NP_004584.1), covering the N-terminal region that includes part of the RRM; this region is highly conserved across human, mouse, and rat, supporting the reported cross-reactivity.
• For Western blotting, note that multiple isoforms may be detected: the canonical isoform migrates at ~34 kDa, while alternative isoforms or cleavage products may appear at ~22 kDa and ~4 kDa. Validate the band pattern in your specific sample type.
• In splicing-related functional studies, consider combining TRA2B antibodies with RNA immunoprecipitation (IP) or immunofluorescence (IF/ICC) to capture its nuclear localization and interaction with target pre-mRNAs.
• While this antibody is listed for ELISA, it is advisable to verify its performance in your specific ELISA setup, as the immunogen is a peptide and may behave differently in solution versus solid-phase formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutions streamlined access to IVD raw materials, with the TRA2B Rabbit mAb being a prime example of our portfolio in transcription and splicing research reagents. From monoclonal antibodies for neuronal disease marker development to bulk ancillary reagents, we can source a wide range of products including validated antibody pairs and rare-target antibodies. Whether you are developing assays for SMN-related disorders or tauopathies, we provide one-on-one technical consulting to accelerate your journey from concept to clinic.