Anti-TPST2 Polyclonal Antibody for WB and ELISA - O60704

Core Product Specifications and Parameters

Biological Background: TPST2 Function and Localization

• Protein-tyrosine sulfotransferase 2 (TPST2), also known as tyrosylprotein sulfotransferase 2, is encoded by the TPST2 gene and belongs to the tyrosylprotein sulfotransferase family. It catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.
• TPST2 is a transmembrane glycoprotein localized in the Golgi apparatus membrane, where it performs post-translational modification of secretory and membrane proteins.
• The protein contains a signal-anchor transmembrane domain and forms disulfide bonds, with three-dimensional structural data available.
• TPST2 is widely expressed across tissues, reflecting its role in general protein sulfation processes.
• UniProt keywords: Golgi apparatus, glycoprotein, disulfide bond, transferase, transmembrane helix, signal-anchor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 280-370 of human TPST2, a region that may be accessible for antibody binding. When designing experiments, consider the native conformation of TPST2 in the Golgi membrane.
• For Western blot (WB) analysis, the predicted molecular weight is approximately 42 kDa; however, glycosylation may affect migration. Validate antibody specificity using positive and negative controls in relevant cell or tissue lysates.
• This antibody is suitable for ELISA applications; consider testing a range of dilutions and using recombinant protein or cell lysates to optimize signal-to-noise ratio.
• Due to cross-reactivity with mouse TPST2, this antibody may be used in mouse model studies; however, verify reactivity in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including validated antibodies like this anti-TPST2 polyclonal antibody. Our portfolio covers a broad range of targets involved in protein modification and enzyme research. Beyond single antibodies, we offer antibody pair development, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, supporting your workflow from discovery to clinical application.