Anti-TPSAB1 Rabbit Polyclonal Antibody for WB and ELISA - Q15661

Core Product Specifications and Parameters

Biological Background: TPSAB1 Function and Localization

• TPSAB1 encodes tryptase alpha/beta-1, also known as tryptase I or tryptase alpha-1, which is the major neutral protease stored in mast cell secretory granules.
• Tryptase is secreted upon mast cell activation and degranulation, and may play a role in innate immunity. Isoform 2 cleaves large substrates like fibronectin more efficiently than isoform 1, but is less efficient toward small substrates.

Related references: PMID:18854315

• The gene undergoes alternative splicing, producing two isoforms (isoform 1 and isoform 2) with distinct substrate specificities and expression patterns.
• At the subcellular level, tryptase is secreted, consistent with its role as an extracellular protease released from mast cells.
• Isoform 1 is predominantly expressed in lung, stomach, spleen, heart, and skin, while isoform 2 is found in aorta, spleen, and breast tumor, particularly in endothelial cells rather than mast cells.
• Key structural features include disulfide bonds, glycosylation, and an N-terminal signal peptide, with the protein being synthesized as a zymogen requiring proteolytic activation.
• The calculated molecular weight is approximately 30.5 kDa (UniProt), often observed at ~31 kDa in denaturing gels due to post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 31–275 of human TPSAB1. Validate antibody specificity by performing a peptide blocking assay or by comparing signals in TPSAB1-overexpressing versus knockdown samples.
• For Western blot, the predicted molecular weight is approximately 31 kDa; however, as a polyclonal antibody, additional bands may be observed. Use appropriate loading controls and consider membrane blocking with 5% non-fat milk.
• For ELISA, optimize coating conditions and antibody concentrations using recombinant TPSAB1 or native protein in biological samples.
• This antibody shows cross-reactivity with mouse and rat, making it suitable for translational studies, but verify reactivity in your specific experimental model.
• Store the antibody at -20°C and avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

As a premier one-stop IVD raw-material partner, CamelBio delivers validated bulk antibodies, optimized polyclonal and monoclonal antibodies, and ancillary reagents to support diagnostic developers and researchers. For studies on mast cell biology, allergy, and innate immunity, the TPSAB1 antibody enables robust detection in human, mouse, and rat models. CamelBio helps streamline your supply chain with quality-controlled materials and customizable sourcing solutions tailored to your project needs—from initial concept to clinical application.