Anti-TPRG1L Rabbit Polyclonal Antibody for WB, ELISA - Q5T0D9

Core Product Specifications and Parameters

Biological Background: TPRG1L Function and Localization

• TPRG1L (Tumor protein p63-regulated gene 1-like protein), also known as MOVER (Mossy fiber terminal-associated vertebrate-specific presynaptic protein) or FAM79A, is a presynaptic protein encoded by the TPRG1L gene in humans.
• It functions as a modulator of synaptic transmission by regulating synaptic release probability, specifically decreasing the calcium sensitivity of neurotransmitter release at presynaptic active zones.
• The protein localizes to the cytoplasmic face of synaptic vesicle membranes and is enriched at the presynaptic active zone, consistent with its role in vesicle fusion dynamics.
• TPRG1L is characterized as a phosphoprotein, and its gene undergoes alternative splicing, suggesting potential regulatory diversity in synaptic function.
• It is associated with mossy fiber terminals of the hippocampus, as indicated by its alternative name, pointing to a role in specific neural circuits.
• The protein is part of the reference proteome and has been identified through proteomics studies, supporting its expression in neural tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–90) of human TPRG1L. For applications requiring the immunizing peptide, this recombinant fragment may be used as a positive control or standard.
• In Western blot assays, a band at approximately 30 kDa is expected. It is advisable to validate the antibody using a known positive sample, such as brain tissue lysate from human, mouse, or rat.
• For ELISA, the recombinant immunogen protein can be utilized to establish a standard curve for quantitative detection, although optimal conditions should be empirically determined.
• Given the cross-reactivity with mouse and rat, this antibody may be suitable for comparative neuroscience studies across rodent models, but expression levels should be verified in the specific tissue or cell type of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, covering every stage from concept to clinic. For neuroscience and synaptic research, this anti-TPRG1L polyclonal antibody exemplifies our commitment to offering validated antibodies and rare target raw-material sourcing. With an extensive portfolio including antibody pairs, bulk ancillary reagents, and customized procurement solutions, CamelBio streamlines your supply chain, empowering your diagnostic and research development.