Anti-TPPP/p25 Rabbit Polyclonal Antibody for WB and ELISA - O94811

Core Product Specifications and Parameters

Biological Background: TPPP/p25 Function and Localization

• TPPP/p25, also known as Tubulin polymerization-promoting protein (p24, p25-alpha), is a 24 kDa microtubule-associated protein encoded by the TPPP gene.
• Plays a critical role in myelination by promoting elongation of the myelin sheath; acts as a microtubule nucleation factor in oligodendrocytes at the Golgi outpost region. Related references: PMID:31522887, PMID:33831707
• Involved in microtubule nucleation, bundling, and stabilization, thereby maintaining the integrity of the microtubule network. Related references: PMID:17105200, PMID:17693641, PMID:18028908
• Exhibits magnesium-dependent GTPase activity; the precise role of this activity remains unclear. Related references: PMID:21316364, PMID:21995432
• Regulates microtubule dynamics by promoting tubulin acetylation through inhibition of the deacetylase HDAC6; phosphorylation by ROCK1 modulates this interaction and influences cell migration. Related references: PMID:20308065, PMID:23093407
• Contributes to cell cycle progression by regulating the G1/S-phase transition. Related references: PMID:23355470
• Participates in astral microtubule organization and mitotic spindle orientation during early mitosis, regulated by LIMK2-mediated phosphorylation. Related references: PMID:22328514
• Localizes to the Golgi outpost, cytoplasm (including microtubule organizing center and spindle), and nucleus; widely expressed across tissues. Post-translational modifications include phosphorylation and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen covers full-length TPPP/p25 (aa 1-219); consider that the polyclonal antibody may recognize multiple epitopes and possible isoforms.
• For Western blot, validate the antibody in lysates from human, mouse, or rat tissues/cells; a band near 24 kDa is expected.
• When performing ELISA, titrate the antibody and use appropriate controls to confirm specificity.
• Given TPPP/p25’s role in microtubule dynamics, consider treatment conditions (e.g., microtubule-stabilizing drugs) that may affect target abundance or localization.
• Always include positive and negative controls relevant to your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, technical services, and consultative support—from concept to clinic. For research on microtubule dynamics and myelination targets like TPPP/p25, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare raw materials. Contact us to streamline your diagnostic development with reliable, high-quality reagents.