Anti-TPP2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P29144

Core Product Specifications and Parameters

Biological Background: TPP2 Function and Localization

• TPP2 is a cytosolic tripeptidyl-peptidase that releases N-terminal tripeptides from polypeptides, acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway (PubMed:25525876, PubMed:30533531). Related references: PMID:25525876 | PMID:30533531
• Plays a critical role in intracellular amino acid homeostasis, ensuring proper amino acid recycling and protein turnover (PubMed:25525876). Related references: PMID:25525876
• Stimulates adipogenesis, promoting fat cell differentiation and lipid accumulation (By similarity).
• Localizes predominantly to the cytoplasm but is also detected in the nucleus, suggesting possible nuclear roles in protein degradation.
• Post-translationally modified by acetylation and phosphorylation, which may regulate its activity or localization.
• Classified as a serine protease and aminopeptidase, belonging to the hydrolase family, and is a known disease-associated variant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-300) of human TPP2, which may target a conserved epitope across species; validate in your experimental system.
• For Western blot, the predicted molecular weight is approximately 138 kDa; consider using appropriate protein ladders and loading controls to confirm band specificity.
• For immunofluorescence/ICC, optimize fixation and permeabilization protocols to detect both cytoplasmic and nuclear localization, as TPP2 is reported in both compartments.
• In ELISA applications, use the antibody as a capture or detection reagent after confirming its performance with recombinant TPP2 protein or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials and technical support. Our TPP2 polyclonal antibody supports studies in the ubiquitin-proteasome pathway and amino acid homeostasis, and we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. For rare or specialized targets like TPP2, CamelBio serves as your reliable sourcing bridge from concept to clinic.