Anti-TPO-R/CD110/c-Mpl Monoclonal Antibody for WB, ELISA - P40238

Core Product Specifications and Parameters

Biological Background: Thrombopoietin Receptor Function and Localization

• The thrombopoietin receptor (TPO-R), encoded by the MPL gene, is the primary regulator of megakaryocyte differentiation and platelet formation, also playing a role in hematopoietic stem cell renewal.
• Upon thrombopoietin (THPO) binding, TPO-R activates JAK2 kinase, leading to tyrosine phosphorylation and recruitment of adaptor proteins including STAT5, SHIP, GRB2, SOS1, and PI3K.
  Related references:
  PMID:15899890
  PMID:37633268
• These signaling cascades drive proliferation, survival, and terminal differentiation of megakaryocytes, ultimately boosting platelet production.
  Related references:
  PMID:15899890
  PMID:37633268
• The receptor exists as multiple isoforms generated by alternative splicing; isoform 1 and isoform 2 are coexpressed in hematopoietic cells.
• Subcellularly, TPO-R localizes to the cell membrane, Golgi apparatus, and cell surface, consistent with its transmembrane signaling role.
• Post-translational modifications reported include glycosylation, phosphorylation, disulfide bond formation, and ubiquitination, as indicated by UniProt keywords.
• Clinically, mutations in MPL are associated with disease variants, underscoring its importance in hematological disorders.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal cytoplasmic domain (aa 536–635); therefore, the antibody can detect both unphosphorylated and activated receptor forms.
• For Western blotting, a single band around 71 kDa is expected; validate with a suitable positive control such as megakaryocytic cell lines (e.g., MEG-01) or primary hematopoietic cells.
• Cross-reactivity with mouse and rat TPO-R makes this antibody suitable for translational research in rodent models; however, confirm reactivity in your specific experimental system.
• For ELISA, optimal coating concentrations and detection conditions should be determined empirically, as performance may vary with sample type and assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive supplier for IVD raw materials, connecting diagnostic developers with validated monoclonal antibodies like this anti-TPO-R clone. In the field of hematopoiesis and platelet biology, we offer ready-to-use antibody pairs, bulk reagent supplies, and customized sourcing of rare-target raw materials to accelerate your assay development. For procurement inquiries or technical consultation, reach out to discuss your project-specific needs.