Anti-TPMT Rabbit Monoclonal Antibody for WB and ELISA - P51580

Core Product Specifications and Parameters

Biological Background: TPMT Function and Localization

• TPMT (Thiopurine S-methyltransferase) is a cytoplasmic enzyme encoded by the TPMT gene, also known as thiopurine methyltransferase.
• It catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine and 6-thioguanine using S-adenosyl-L-methionine as a methyl donor, modulating their cytotoxic effects. Related references: PMID:18484748 PMID:657528
• A natural endogenous substrate for TPMT has not been identified; its primary characterized role is in xenobiotic metabolism.
• TPMT is classified as a methyltransferase and transferase, with a molecular weight of approximately 28.2 kDa.
• The enzyme localizes to the cytoplasm.
• Post-translational modifications include acetylation and phosphorylation, and the protein's three-dimensional structure has been determined.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region within human TPMT (amino acids 50-150), which may contribute to the observed cross-reactivity with mouse and rat orthologs. When working with these species, validate the antibody in the target tissue or cell line.
• For Western blotting, expect a band near 28 kDa; optimize sample preparation and antibody dilution for your specific system.
• In ELISA, consider using the antibody as a capture or detection reagent, and titrate to determine optimal concentrations based on your coating antigen and detection method.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material sourcing, offering a broad portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers and developers focused on thiopurine drug metabolism and pharmacogenomics, this anti-TPMT antibody can be integrated into custom assay development. Additionally, CamelBio supports the procurement of rare-target raw materials, helping you streamline your supply chain from concept to clinic.