Anti-TPM2 Rabbit Polyclonal Antibody - P07951

Core Product Specifications and Parameters

Biological Background: TPM2 Function and Localization

• TPM2 encodes tropomyosin beta chain, an actin-binding coiled-coil protein that regulates muscle contraction and cytoskeletal dynamics.
• In striated muscle, TPM2 associates with the troponin complex to confer calcium-sensitive control of actin-myosin interaction.
• Smooth muscle contraction is modulated by TPM2 binding to caldesmon, an actin- and calmodulin-binding protein.
• Non-muscle TPM2 isoforms stabilize cortical actin filaments and may facilitate agonist-mediated receptor internalization.
• The protein localizes predominantly to the cytoplasmic cytoskeleton.
• Post-translational acetylation and phosphorylation have been identified, potentially modulating TPM2 function.
• Alternative splicing yields tissue-specific isoforms, contributing to functional diversity.
• Mutations in TPM2 are linked to nemaline myopathy and other congenital myopathies (disease variant).
• TPM2 is detected in primary breast cancer tissue but absent from normal breast, suggesting a role in oncogenic processes.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-100) of human TPM2; verify recognition of specific isoforms in your sample system.
• For Western blotting, a band near 33 kDa is expected; additional bands may appear due to isoforms or post-translational modifications.
• In immunohistochemistry (IHC-P) and immunocytochemistry (IF/ICC), test antigen retrieval methods and antibody dilutions empirically.
• For immunoprecipitation (IP), consider covalent coupling to avoid heavy-chain interference.
• This polyclonal antibody recognizes human, mouse, and rat TPM2; cross-reactivity with other species should be validated.
• Confirm specificity using positive/negative controls (e.g., tissues expressing TPM2 or siRNA knockdown lysates).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes seamless access to a wide portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. Our team supports projects from early-stage assay development through clinical validation, covering targets like TPM2 in the fields of muscle biology and cytoskeleton research. For bulk or custom-sourced antibodies and rare-target reagents, CamelBio provides reliable procurement solutions to accelerate your diagnostic workflows.