Anti-TPK1 Polyclonal Antibody for WB and ELISA - Q9H3S4

Core Product Specifications and Parameters

Biological Background: TPK1 Function and Localization

• TPK1 encodes thiamine pyrophosphokinase 1, also known as placental protein 20 (PP20), belonging to the thiamine pyrophosphokinase family.
• It catalyzes the conversion of thiamine to thiamine pyrophosphate (TPP) using UTP, linking TPP biosynthesis to pyrimidine metabolism. Related references: PMID:38547260
• TPP is an essential cofactor for mitochondrial pyruvate dehydrogenase; therefore, TPK1 indirectly regulates pyruvate oxidation and lipogenesis. Related references: PMID:38547260
• In vitro, TPK1 can also utilize ATP or CTP as phosphate donors, albeit with significantly lower efficiency, suggesting UTP is the physiological substrate. Related references: PMID:11342111, PMID:38547260
• TPK1 is expressed in heart, kidney, testis, small intestine, and peripheral blood leukocytes, with low levels detected in various tissues.
• The protein contains an ATP-binding domain and exhibits kinase/transferase activity.
• Alternative splicing produces multiple transcript variants.
• Mutations in TPK1 are associated with thiamine metabolism dysfunction syndrome 5 (THMD5), a neurodegenerative disorder.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 243 amino acids; consider this epitope region when designing controls or cross-reactivity experiments.
• For Western blot, expect a band near 27 kDa; validate specificity using relevant positive and negative cell lysates.
• In ELISA, optimize coating antigen and antibody titration for your specific sample type and detection system.
• As a polyclonal antibody, it may recognize multiple epitopes; consider knockout or knockdown validation for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw material sourcing, offering a comprehensive portfolio from validated antibodies to ancillary reagents. This anti-TPK1 antibody supports research into thiamine metabolism and related disorders, and CamelBio ensures seamless access to high-quality monoclonal/polyclonal antibodies, antigen pairs, and custom development services to accelerate your diagnostic projects from concept to clinic.