Anti-TPI1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA - P60174

Core Product Specifications and Parameters

Biological Background: TPI1 Function and Localization

• Triosephosphate isomerase (TPI1) is a highly efficient metabolic enzyme that catalyzes the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.
• TPI1 also exhibits methylglyoxal synthase activity, producing the reactive cytotoxic side-product methylglyoxal, which can modify proteins, DNA, and lipids.
• The enzyme is predominantly localized in the cytoplasm, consistent with its central role in energy metabolism.
• TPI1 is subject to multiple post-translational modifications, including acetylation, methylation, nitration, phosphorylation, and isopeptide bond formation, which may regulate its function.
• Mutations in TPI1 are associated with hereditary hemolytic anemia, underscoring its importance in erythrocyte metabolism.
• Alternative promoter usage and splicing generate multiple transcript variants of the TPI1 gene, contributing to regulatory diversity.

Experimental Guidance and Technical Tips

• The full-length immunogen (aa 1-249) elicits a polyclonal response, potentially recognizing multiple epitopes across human, mouse, and rat TPI1.
• For Western blot, expect a band at approximately 27 kDa; consider using whole-cell lysates from tissues or cell lines with high glycolytic activity.
• In immunohistochemistry and immunofluorescence, optimal fixation and permeabilization protocols should be determined empirically for the target tissue.
• ELISA performance may depend on coating buffer and detection system; titrate the antibody to achieve the desired signal-to-noise ratio.
• Cross-reactivity with other TPI family members is unlikely but should be evaluated in the relevant experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers with validated IVD raw materials, including optimized polyclonal antibodies like this anti-TPI1 antibody, to support metabolic enzyme research and assay development. Our sourcing network covers validated antibody pairs, bulk ancillary reagents, and rare target procurement, helping you move confidently from concept to clinic.