Anti-TPH2 Polyclonal Antibody for WB, ELISA - Q8IWU9

Core Product Specifications and Parameters

Biological Background: TPH2 Function and Localization

• Tryptophan 5-hydroxylase 2 (TPH2) is also known as neuronal tryptophan hydroxylase or tryptophan 5-monooxygenase 2.
• TPH2 catalyzes the rate-limiting step in serotonin biosynthesis from tryptophan, a pathway critical for brain function.
• It is a monooxygenase enzyme that requires iron as a cofactor for catalytic activity.
• The protein is subject to phosphorylation, which may regulate its enzymatic activity and subcellular localization.
• Alternative splicing generates multiple isoforms, and RNA editing events have been reported.
• Disease-associated variants of TPH2 have been identified, linking the enzyme to neurological and psychiatric disorders.
• TPH2 is specifically expressed in the brain, consistent with its role in neuronal serotonin production.
• Three-dimensional structures of TPH2 provide insights into its catalytic mechanism and inhibitor binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-170 of human TPH2, an N-terminal region; consider verifying antibody reactivity with the full-length native protein in brain tissue lysates.
• For western blotting, a specific band near 56 kDa is expected; validate the antibody in relevant human, mouse, or rat brain samples, and consider using positive and negative controls.
• When setting up ELISA, optimize the antibody as capture or detection reagent in combination with a matched antibody pair; performance may vary depending on sample matrix and target concentration.
• Because the antibody is polyclonal, it may recognize multiple epitopes and possibly related isoforms; cross-reactivity with other tryptophan hydroxylases or proteins should be assessed in the experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to IVD raw materials, technical services, and consulting support from concept to clinic. This anti-TPH2 polyclonal antibody is well suited for research into serotonin biosynthesis and neurological disease mechanisms. In addition to antibodies, CamelBio can source validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and rare-target raw materials to accelerate your IVD development and procurement.