Anti-TPH1 Rabbit Monoclonal Antibody for IHC-P, ELISA - P17752

Core Product Specifications and Parameters

Biological Background: Tryptophan Hydroxylase 1 (TPH1) Function and Localization

• Tryptophan hydroxylase 1 (TPH1) is a key enzyme in serotonin biosynthesis, catalyzing the rate-limiting step that converts L-tryptophan to 5-hydroxy-L-tryptophan.
• Encoded by the TPH1 gene (also known as TPH, TPRH, TRPH) in humans, with alternative splicing producing multiple isoforms.
• Isoform 1 is the primarily studied form, while the isoform represented by this entry appears to have a more restricted tissue expression pattern.
• TPH1 is a monooxygenase and oxidoreductase that requires iron as a metal cofactor for catalytic activity.
• The protein is subject to post-translational modifications including phosphorylation and ubiquitin conjugation, which may regulate its activity and stability.
• Structural studies have provided insights into the enzyme's active site and catalytic mechanism.
• As the rate-limiting enzyme for serotonin synthesis, TPH1 plays a critical role in serotonergic signaling pathways.
• TPH1 has been identified in proteomics screens and is cataloged as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 102-402) of human TPH1, which is expected to generate antibodies recognizing multiple isoforms due to high sequence conservation within this domain. Consider suitability for detecting various TPH1 variants.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-F/IF-P), optimize antigen retrieval and permeabilization based on the tissue type and preparation method to ensure adequate antibody penetration.
• This monoclonal antibody is suitable for ELISA applications; consider direct or indirect detection methods depending on assay sensitivity requirements.
• Cross-reactivity with mouse and rat TPH1 has been noted; validate in the specific experimental model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop supplier of IVD raw materials, offering validated antibody pairs, optimized monoclonals, and bulk ancillary reagents to support your diagnostic development. This anti-TPH1 antibody exemplifies our commitment to providing high-quality reagents for serotonin pathway research and related applications. Leverage our expertise to streamline your sourcing from concept to clinic.