Anti-TPH1 Polyclonal Antibody for WB, ELISA - P17752

Core Product Specifications and Parameters

Biological Background: TPH1 Function and Localization

• TPH1 (Tryptophan 5-hydroxylase 1) is the rate-limiting enzyme in serotonin biosynthesis, catalyzing the conversion of L-tryptophan to 5-hydroxy-L-tryptophan.
• It functions as an iron-dependent monooxygenase, requiring metal binding for catalytic activity.
• The protein is subject to alternative splicing, producing multiple isoforms with distinct expression patterns.
• Post-translational modifications include phosphorylation and ubiquitination (Ubl conjugation), which may regulate enzyme activity and turnover.
• TPH1 appears less widely expressed than isoform 1, suggesting a restricted tissue distribution.
• It plays a key role in peripheral serotonin production, with implications for vascular, gastrointestinal, and metabolic functions.
• The calculated molecular weight is ~51 kDa, consistent with its 444-amino acid sequence.
• 3D structural data are available, aiding in understanding active site architecture and inhibitor design.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 345–444 near the C-terminus; this polyclonal antibody may detect multiple epitopes within this region.
• For Western blotting, a band near 51 kDa is expected in human, mouse, or rat lysates; consider optimizing sample preparation (e.g., denaturing conditions) and antibody dilution.
• Cross-reactivity with mouse and rat TPH1 suggests the epitope is conserved, supporting multi-species applications; validate reactivity in each species’ relevant tissue or cell system.
• For ELISA, test both direct and sandwich formats, and empirically determine coating antigen concentration and antibody dilution.
• As a polyclonal reagent, lot-to-lot consistency should be monitored in quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner offering this TPH1 antibody alongside a comprehensive portfolio of validated antibody pairs, optimized monoclonals and polyclonals, and bulk ancillary reagents. Whether you are developing serotonin-related diagnostic kits or researching neuroendocrine disorders, our one-stop sourcing model—from target selection to clinical-scale supply—streamlines your supply chain for rare and routine targets alike.