Anti-TPD52L3 Polyclonal Antibody for WB, IF-P, ELISA - Q96J77

Core Product Specifications and Parameters

Biological Background: TPD52L3 Function and Localization

• TPD52L3, also known as D55, hD55, TPD55, and NYD-SP25, is a testis-specific protein belonging to the tumor protein D52-like family.
• The gene undergoes alternative splicing, yielding multiple transcript isoforms that may contribute to functional diversity.
• It is highly expressed in adult testis, showing 5.6-fold higher levels than in fetal testis, suggesting a role in testis development and maturation.
• The protein contains a coiled-coil domain, potentially facilitating protein-protein interactions critical for its function.
• UniProt annotates TPD52L3 as a proteomics-identified component of the reference proteome, confirming its endogenous expression in human tissues.
• The predicted molecular weight is approximately 15.5 kDa (UniProt), consistent with the observed 16 kDa band in Western blotting.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-132), which includes the coiled-coil domain; consider its potential impact on epitope accessibility in native versus denaturing conditions.
• For Western blot, expect a single band near 16 kDa in human, mouse, and rat testis lysates; validate specificity with appropriate positive and negative controls.
• In immunofluorescence (IF-P), optimize permeabilization and include a nuclear counterstain; testis tissue sections are recommended as the primary positive control.
• For ELISA, the polyclonal nature allows pairing with a monoclonal capture antibody if needed; titrate both antibodies to determine optimal sandwich conditions.
• Cross-reactivity with mouse and rat is reported; however, confirm reactivity in your specific experimental system before large-scale studies.

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CamelBio connects diagnostic manufacturers, laboratories, and research institutes with high-quality IVD raw materials, including validated polyclonal antibodies for targets like TPD52L3. We streamline procurement from concept to clinic, offering bulk reagents, optimized antibody pairs, and tailored technical support to accelerate your assay development in reproductive biology and oncology. Let CamelBio be your reliable partner for rare-target sourcing and seamless supply chain solutions.