Anti-tPA/PLAT Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - P00750

Core Product Specifications and Parameters

Biological Background: tPA/PLAT Function and Localization

• Tissue-type plasminogen activator (tPA), encoded by the PLAT gene, is a secreted serine protease that converts plasminogen to plasmin, a key enzyme in fibrinolysis and tissue remodeling.
• tPA plays essential roles in cell migration, tissue degradation, and various physiopathological events, including participation in the block to polyspermy during oocyte activation.
• It is synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, tears, seminal fluid, and milk.
• Subcellularly, tPA localizes to the extracellular space, consistent with its function as a secreted protease.
• The protein contains multiple structural domains: EGF-like domain, kringle domains, and a serine protease domain, characteristic of the plasminogen activator family.
• Post-translational modifications include disulfide bonds and glycosylation, and it is produced as a zymogen that requires proteolytic cleavage for activation.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide spanning amino acids 463–562 of human tPA, a region that may facilitate strong reactivity across human, mouse, and rat species.
• For Western blot experiments, note the predicted molecular weight of approximately 63 kDa; recommend using appropriate lysis buffers and reducing conditions.
• In immunofluorescence and immunocytochemistry applications, consider fixation and permeabilization protocols optimized for secreted proteins.
• ELISA applications may be compatible with both purified recombinant tPA and biological fluid samples; pilot testing is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target procurement to support every stage from concept to clinic. This tPA/PLAT monoclonal antibody is ideal for research in fibrinolysis, tissue remodeling, and related diagnostic development. Contact us to streamline your supply chain and accelerate your next project.