Anti-TP73 Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - O15350

Core Product Specifications and Parameters

Biological Background: TP73 Function and Localization

• Tumor protein p73, also known as p53-like transcription factor or p53-related protein, is encoded by the TP73 gene and belongs to the p53 family.
• Functions in the apoptotic response to DNA damage; isoforms containing the transactivation domain promote apoptosis, while those lacking it act anti-apoptotically and inhibit p53 and transactivating p73 isoforms.
• Acts as a tumor suppressor and an activator of FOXJ1 expression, essential for the positive regulation of lung ciliated cell differentiation. Related references: PMID:34077761
• Localizes to both the nucleus and cytoplasm.
• Expressed in various tissues including brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus, and pancreas; highly expressed in fetal tissue and respiratory epithelium. Related references: PMID:34077761
• Keywords indicate involvement in transcription regulation, cell cycle, apoptosis, and roles as a tumor suppressor; subject to alternative splicing and promoter usage, with metal-binding and zinc finger domains.
• Phosphoprotein with potential isopeptide bond and Ubl conjugation, reflecting complex post-translational regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 487-636) of human TP73, which may recognize both transactivation-competent and -deficient isoforms, but validation in the relevant isoform context is advised.
• For Western blotting, the predicted molecular weight is approximately 70 kDa; use a protein ladder and positive control lysates (e.g., HeLa, MCF7) to confirm the band.
• For immunofluorescence and immunohistochemistry, anticipate nuclear and cytoplasmic staining patterns; permeabilization and blocking conditions should be optimized for each sample type.
• In ELISA applications, titration of both antibody and antigen is recommended to establish optimal signal-to-noise ratios; the antibody can be explored for both direct and sandwich ELISA formats.
• Cross-reactivity with mouse is indicated; for mouse tissue or cell lines, validate under your specific experimental conditions to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated access to high-quality IVD raw materials, supporting diagnostic manufacturers and researchers from early development to clinical application. This anti-TP73 polyclonal antibody is part of our extensive portfolio for oncology and ciliopathy research, complemented by our capability to source or produce validated antibody pairs, bulk reagents, and rare target materials. Partner with us to streamline your pipeline with reliable, one-stop procurement and technical support.