Anti-TP53RK Rabbit Polyclonal Antibody for WB and ELISA - Q96S44

Core Product Specifications and Parameters

Biological Background: TP53RK Function and Localization

• Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that recognize codons beginning with adenine.\nRelated references:\nPMID:22912744, PMID:27903914
• Possesses ATPase activity in the context of the EKC/KEOPS complex and likely supports the catalytic subunit OSGEP in the threonylcarbamoyl transfer reaction.
• Acts as an atypical serine/threonine protein kinase, directly phosphorylating Ser-15 of the p53/TP53 tumor suppressor protein, potentially contributing to its activation.\nRelated references:\nPMID:11546806
• Localizes to both the cytoplasm and nucleus, consistent with its roles in tRNA modification and transcriptional regulation.
• Highly expressed in testis, with weaker expression observed in heart, kidney, and spleen, indicating tissue-specific regulatory functions.
• Belongs to the Bud32 family of atypical kinases and is associated with diseases such as epilepsy and intellectual disability (GAMOS4), highlighting its clinical relevance.
• Annotated with keywords including ATP-binding, kinase, transferase, tRNA processing, and phosphoprotein, reflecting its enzymatic and regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human TP53RK (aa 1–253), which typically yields antibodies recognizing both native and denatured protein, making it suitable for WB under reducing conditions.
• For ELISA, consider employing the recombinant immunogen as a positive control to establish assay sensitivity and specificity.
• Confirm cross-reactivity in mouse and rat lysates by detecting a band at approximately 28 kDa; include appropriate tissue or cell line controls.
• Given its dual subcellular localization, incorporate cytoplasmic and nuclear fractionation controls to accurately assess TP53RK distribution in your sample system.
• Validate antibody specificity using peptide-blocking assays or siRNA/knockout cell lines, especially when evaluating phosphorylation-dependent functions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner for diagnostic manufacturers and research laboratories worldwide. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents across diverse disease areas, including oncology and genetic disorders. For TP53RK-related research and assay development, our team can assist with rare target raw-material procurement, custom antibody generation, and technical consulting to support your project from concept to clinic.