Anti-TP53BP2 Rabbit Polyclonal Antibody for IHC-P and ELISA - Q13625

Core Product Specifications and Parameters

Biological Background: TP53BP2 Function and Localization

• Encoded by the TP53BP2 gene, apoptosis-stimulating of p53 protein 2 (ASPP2) is a 126 kDa protein also referred to as Bbp, 53BP2, or p53BP2.
• Enhances the DNA binding and transactivation activity of TP53 on proapoptotic gene promoters, thereby promoting apoptosis. Related references: PMID:12524540
• Inhibits NAE1-mediated conjugation of NEDD8 to CUL1, decreasing NAE1-driven apoptosis.
• Regulates cell cycle progression by impeding the G2/M transition.
• Localizes to the cytoplasm, perinuclear region, and nucleus.
• Widely expressed in tissues, with high levels in spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood leukocytes; expression is reduced in breast carcinomas harboring wild-type TP53 and elevated in lung cancer cell lines.
• Contains structural motifs including ANK repeats and an SH3 domain, and is regulated by phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 360–600 within the central region of human TP53BP2, which may recognize multiple splice variants and orthologs; cross-reactivity with mouse and rat is reported.
• For IHC-P, consider optimizing antigen retrieval protocols and validating in formalin-fixed, paraffin-embedded tissue sections known to express TP53BP2, such as spleen or lung tumor samples.
• When performing ELISA, ensure the coating antigen contains the relevant epitope region, and include appropriate negative and positive controls to confirm specificity.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, supporting projects from concept to clinic. For apoptosis and cell cycle research, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, empowering your assay development. Our sourcing network also facilitates procurement of rare-target raw materials, ensuring you find the right components for your diagnostic or research applications.