Anti-TP53AIP1 Rabbit Polyclonal Antibody for WB, ELISA - Q9HCN2

Core Product Specifications and Parameters

Biological Background: TP53AIP1 Function and Localization

• TP53AIP1 (p53-regulated apoptosis-inducing protein 1) is a 13 kDa protein encoded by the TP53AIP1 gene, also known as P53AIP1.
• It is a crucial mediator of p53/TP53-dependent apoptosis, playing a key role in programmed cell death triggered by DNA damage and tumor suppression.
• The protein is localized exclusively to the mitochondrion, where it likely participates in the intrinsic apoptotic pathway through interactions with mitochondrial membrane components.
• TP53AIP1 expression is highly tissue-specific; it has been detected exclusively in the thymus, suggesting a specialized role in immune cell development and selection.
• Multiple transcript variants arise from alternative splicing, as indicated by the keyword “Alternative splicing,” though their functional significance remains to be fully elucidated.
• The protein is part of the reference proteome for Homo sapiens, underscoring its well-characterized sequence and functional annotation in the Swiss-Prot database.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal amino acids 1–108 of human TP53AIP1, which may provide broad recognition of full-length protein and splice variants.
• For Western blotting (WB), consider a starting dilution range (e.g., 1:500–1:2000) and optimize based on sample abundance and detection system.
• When using ELISA, titrate the antibody against the recombinant immunogen or cell lysates to determine optimal coating and detection conditions.
• Verify cross-reactivity with mouse and rat samples in your specific experimental context, as antibody performance can vary across species and tissue types.
• As a polyclonal antibody, note that lot-to-lot consistency should be assessed; consider validation with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For p53-regulated apoptosis studies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support in sourcing rare target raw materials to streamline your assay development and biomarker research.