Anti-TOX Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O94900

Core Product Specifications and Parameters

Biological Background: TOX Function and Localization

• TOX is a DNA-binding transcriptional regulator that recognizes GC-rich sequences near transcription start sites, potentially altering chromatin structure to modulate transcription factor access.
• It plays a critical dual role in neural development, maintaining neural stem cell proliferation and promoting neurite outgrowth in migrating cortical neurons, with downstream targets including SOX2, EOMES, and ROBO2.
• TOX is essential for lymphoid tissue-inducer (LTi) cell development and subsequent formation of peripheral lymph nodes and Peyer's patches.
• It acts as a developmental checkpoint in thymocyte positive selection, driving commitment to the T cell lineage and differentiation of diverse T cell subsets—including CD4+ helper, CD8+ cytotoxic, regulatory T, and CD1d-dependent NKT cells.
• TOX specifies natural killer (NK) cell lineage commitment at the bone marrow progenitor stage. Related references: PMID:21126536
• In chronic antigen stimulation, TOX promotes T cell exhaustion by inducing inhibitory receptors like PDCD1, diverting T cell fate from effector/memory toward exhaustion to prevent activation-induced cell death.
• The protein is localized to the nucleus and is subject to ubiquitin-like conjugation (Ubl conjugation). Keywords include chromatin regulator, DNA-binding, neurogenesis, transcription, and proteomics identification.
• TOX shows elevated expression at the protein level in tumor-infiltrating CD8+ T cells, suggesting a role in the tumor immune microenvironment. Related references: PMID:31207604

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 30–165 of human TOX (NP_055544.1), covering an N-terminal region; consider that this polyclonal antibody may recognize full-length TOX and potential splice variants.
• For Western blot (WB), expect a band at approximately 58 kDa. Validate in the relevant sample system (human, mouse, rat) using appropriate positive and negative controls.
• For immunohistochemistry (IHC-P), antigen retrieval methods may influence staining; optimize conditions for your tissue type and include isotype controls. Nuclear localization should be observed.
• For immunofluorescence/immunocytochemistry (IF/ICC), confirm nuclear staining and counterstain with DAPI to verify localization.
• As a polyclonal antibody, lot-to-lot variability can occur; we recommend validating each new lot in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, from validated antibody pairs and recombinant proteins to ancillary reagents. TOX is an emerging target in immune-oncology and neurobiology, and our portfolio includes optimized antibodies for demanding applications. Whether you need bulk quantities or rare-target sourcing, CamelBio accelerates your development pipeline from concept to clinic.