Anti-TORC2/CRTC2 Rabbit Monoclonal Antibody for WB and ELISA - Q53ET0

Core Product Specifications and Parameters

Biological Background: TORC2 Function and Localization

• Protein identity and aliases: TORC2, also known as CREB-regulated transcription coactivator 2 (CRTC2) or transducer of CREB protein 2, is encoded by the CRTC2 gene in humans. It belongs to the CRTC family of transcriptional coactivators.
• Core transcriptional coactivator function: TORC2 acts as a potent coactivator for CREB1, enhancing CREB1 interaction with TAF4 and activating transcription through both consensus and variant cAMP response element (CRE) sites. Its activity is regulated by the SIK/TORC signaling pathway; it is active when dephosphorylated and can function independently of CREB1 Ser-133 phosphorylation.
• Metabolic regulation: As a key component of the LKB1/AMPK/TORC2 signaling pathway, TORC2 regulates hepatic gluconeogenesis. It also serves as a coactivator of PPARGC1A, inducing mitochondrial biogenesis in muscle cells, thereby linking energy metabolism and gene expression.
• Subcellular localization: TORC2 shuttles between the cytoplasm and the nucleus, a process tightly controlled by phosphorylation and cellular signals, allowing rapid modulation of CREB-dependent transcription.
• Tissue distribution and disease relevance: Widely expressed, with highest levels in the thymus, muscle, spleen, ovary, and lung; lower in liver and pancreas. Highly expressed in HEK293T cells and insulinomas, and present in both B and T lymphocytes. This pattern underscores its role in immune function, metabolism, and hormone regulation.
• Post-translational modifications and interactions: Multiple modifications (acetylation, methylation, phosphorylation, isopeptide bond formation, and ubiquitination) fine-tune TORC2 activity, stability, and localization. It also participates in host-virus interactions, coactivating TAX-mediated HTLV-1 LTR transcription.

Experimental Guidance and Technical Tips

• The immunogen for this antibody corresponds to a peptide within amino acids 594–693 of human TORC2. When designing epitope-specific reagents or confirming recognition, consider mapping the binding region and validating in the relevant sample system.
• This antibody is suitable for Western blot (predicted band ~73 kDa) and ELISA. For WB, ensure proper negative controls and consider phospho-regulation of TORC2, which may affect detection or band pattern.
• Given cross-reactivity with rat, the antibody can be used for comparative studies across human and rat samples. Verify specificity in tissue or cell types with known TORC2 expression (e.g., thymus, muscle, insulinoma cells).
• For functional studies (e.g., CREB transcription assays), reagents targeting TORC2 post-translational modifications may be required in parallel to fully assess activation state.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials and technical support, streamlining development from concept to clinic. For transcription and metabolic regulation projects, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents tailored to your assay needs. Whether you need standardized research-use products or assistance sourcing rare targets like TORC2, our platform bridges the gap between discovery and reliable supply.