Anti-Topors Rabbit Polyclonal Antibody for WB and ELISA - Q80Z37

Core Product Specifications and Parameters

Biological Background: Topors Function and Localization

• Topors is an E3 ubiquitin-protein ligase and E3 SUMO1-protein ligase, functioning as a probable tumor suppressor involved in regulating cell growth, proliferation, and apoptosis.
• It regulates p53/TP53 stability through ubiquitin-dependent degradation, linking it to critical tumor suppressor pathways.
• May regulate chromatin modification through sumoylation of several chromatin modification-associated proteins, impacting gene expression and genome integrity.
• May be involved in DNA-damage-induced cell death through IKBKE sumoylation, suggesting a role in the DNA damage response.
• Localizes to the nucleus and PML bodies, consistent with its functions in protein modification and chromatin regulation.
• Contains a RING-type zinc finger domain, characteristic of E3 ligases, and is classified as a metal-binding phosphoprotein involved in ubiquitin and ubiquitin-like conjugation pathways.
• Also known as LUN, TP53BPL, p53BP3, and topoisomerase I-binding RING finger protein, reflecting its historical associations with tumor suppression and topoisomerase I interaction.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from mouse Topors (amino acids 100-200); due to high sequence conservation, this antibody is expected to recognize human Topors. Validation in the relevant human sample system is recommended.
• For Western blotting, consider optimizing antibody dilution and detection conditions, as polyclonal antibodies may exhibit lot-to-lot variability.
• In ELISA, the antibody may be used as either capture or detection reagent; pairwise testing with antigen is advised to confirm performance.
• Since the antibody cross-reacts with mouse and rat, it is suitable for studies involving these species; however, confirm specificity in the chosen model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Topors polyclonal antibody supports research into tumor suppression, p53 regulation, and DNA damage response pathways. For your assay development needs, we can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist with sourcing rare target raw materials. Contact us to streamline your IVD creation process.