Anti-Topo IIα Polyclonal Antibody for WB, IHC-P, ELISA - P11388

Core Product Specifications and Parameters

Biological Background: Topo IIα Function and Localization

• DNA topoisomerase 2-alpha (Topo IIα), encoded by the TOP2A gene, is a critical ATP-dependent enzyme that modulates DNA topology by transiently cleaving and religating double-stranded DNA, allowing the passage of one duplex through another (PubMed:17567603, PubMed:18790802, PubMed:22013166). Related references: PMID:17567603 PMID:18790802 PMID:22013166
• Topo IIα is predominantly localized to the nucleus, with enrichment in the nucleoplasm and nucleolus; it is also detected in the cytoplasm, particularly during cell division.
• It is widely expressed in proliferative tissues such as tonsil, spleen, lymph node, thymus, and testis, and is notably overexpressed in high-grade lymphomas, squamous cell lung tumors, and seminomas (PubMed:9155056). Related references: PMID:9155056
• Post-translational modifications, including phosphorylation, acetylation, and ubiquitination, regulate Topo IIα activity, stability, and cellular localization.
• The protein exhibits alternative splicing, yielding multiple isoforms with molecular weights in the range of 174 to 182 kDa.
• Topo IIα may also participate in circadian rhythm regulation by influencing the period length of BMAL1 transcriptional oscillation (By similarity).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 1431-1531), which may provide high specificity for Topo IIα.
• For Western blot, due to the large protein size (~174-182 kDa), optimize protein transfer conditions (e.g., lower methanol percentage, extended transfer time) and verify bands with appropriate molecular weight standards.
• In immunohistochemistry (IHC-P), consider antigen retrieval protocols (e.g., heat-induced epitope retrieval) and titrate the antibody to minimize background.
• For ELISA applications, this polyclonal antibody can be used as a capture or detection reagent; pairing with a monoclonal antibody may enhance specificity in sandwich assays.
• Cross-reactivity is validated for human samples; for other species, sequence homology of the immunogen should be checked before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to high-quality IVD raw materials, including validated antibody pairs, recombinant proteins, and bulk ancillary reagents. This Topo IIα polyclonal antibody is part of our extensive portfolio supporting cancer diagnostics and cell biology research. Our team facilitates rapid sourcing of rare targets and customized raw material solutions to accelerate your assay development from concept to clinic.