Anti-TOP2A (DNA Topoisomerase II Alpha) Rabbit Polyclonal Antibody for WB and ELISA - P11388

Core Product Specifications and Parameters

Biological Background: DNA Topoisomerase II Alpha Function and Localization

• DNA topoisomerase 2-alpha (TOP2A) is a nuclear enzyme essential for resolving topological problems in DNA such as supercoiling, catenation, and decatenation, critical during DNA replication, transcription, and chromosome segregation. Related references: PMID:17567603, PMID:18790802, PMID:22013166
• TOP2A forms a homodimer and catalyzes the ATP-dependent passage of one double-stranded DNA segment through a transient double-stranded break in another, altering DNA topology via a cleavage-and-religation mechanism.
• Belongs to the type II DNA topoisomerase family and is highly conserved among eukaryotes; it shares homology with TOP2B, the beta isoform, but exhibits distinct expression patterns and functional roles.
• Localizes primarily to the nucleus, including nucleoplasm and nucleolus, and is also detected in the cytoplasm during certain cell cycle phases.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, which modulate protein stability, enzymatic activity, and chromosome binding.
• Expressed in various normal tissues such as tonsil, spleen, lymph node, thymus, and testis, and highly expressed in proliferating cells; its expression is upregulated in many cancers, including high-grade lymphomas and squamous cell lung tumors. Related references: PMID:9155056
• Plays a putative role in circadian rhythm regulation by influencing BMAL1 transcriptional oscillation.
• Often used as a proliferation marker and therapeutic target in oncology; inhibitors like etoposide specifically target TOP2A to induce DNA damage in rapidly dividing cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide near the C-terminus of human TOP2A; the antibody may recognize both full-length and truncated isoforms if present.
• For Western blot, a band of approximately 174 kDa is expected in human cell or tissue lysates; use a high-molecular-weight range gel and appropriate transfer conditions.
• Consider enriching nuclear fractions to enhance detection, as TOP2A is predominantly nuclear.
• For ELISA, the synthetic peptide can be immobilized as a positive control; the antibody can capture native TOP2A in coated sample wells.
• Since this is a polyclonal antibody, lot-to-lot consistency should be verified for quantitative applications.
• Validate antibody specificity by siRNA knockdown or comparison with TOP2A-negative cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated antibodies, antigens, and ancillary reagents, to support assay development and manufacturing. For cancer biomarker and cell proliferation research, this anti-TOP2A antibody complements our catalog of oncology-related raw materials. From bulk polyclonal and monoclonal antibodies to rare target sourcing, we help diagnostic manufacturers and CROs streamline procurement and scale up production.