Anti-TOP1MT Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q969P6

Core Product Specifications and Parameters

Biological Background: TOP1MT Function and Localization

• TOP1MT encodes mitochondrial DNA topoisomerase I, a key enzyme responsible for relieving supercoiling and torsional tension during mitochondrial DNA replication and transcription (UniProtKB: Q969P6).
• The enzyme functions by transiently cleaving one DNA strand via a transesterification reaction, forming a covalent DNA-(3'-phosphotyrosyl)-enzyme intermediate, allowing the free strand to rotate and resolve supercoils before religation (By similarity).
• TOP1MT is localized exclusively to the mitochondrion, where it plays a critical role in maintaining mitochondrial DNA topology.
• Expression is ubiquitous, with highest levels observed in skeletal muscle, heart, brain, and fetal liver.
• As a type IB topoisomerase, TOP1MT contains a DNA-binding domain and a catalytic tyrosine residue essential for its activity; keywords include isomerase, DNA-binding, and topoisomerase.
• Alternative splicing of the TOP1MT gene produces multiple transcript variants, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 470-550) of human TOP1MT, suggesting the antibody may detect full-length and C-terminal-containing isoforms; verify cross-reactivity with rat isoforms by sequence alignment.
• For Western blotting, an expected band near 70 kDa is typical; optimize transfer for high-molecular-weight proteins and use mitochondrial-enriched samples for better signal.
• In immunohistochemical analysis (IHC-P), anticipate mitochondrial staining patterns; include appropriate controls and optimize antigen retrieval for mitochondrial antigens.
• Validate antibody specificity using knockout or knockdown models to minimize potential cross-reactivity with nuclear topoisomerases like TOP1.
• For ELISA, the antibody can bind recombinant or purified TOP1MT; consider pairing with a matched detection antibody for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, technical services, and consulting to support diagnostic developers from concept to clinic. This anti-TOP1MT antibody is a valuable tool for mitochondrial function and disease research, and can be integrated into assay development workflows. Beyond antibodies, CamelBio offers validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to streamline your project needs.