Anti-TOMM7 Rabbit Polyclonal Antibody for WB, ELISA - Q9P0U1

Core Product Specifications and Parameters

| Product Name | TOMM7 Rabbit pAb | | Remarks/Alias | TOM7; TOMM7 | | Species | Human | | Gene ID (Human) | 54543 | | Gene ID | 54543 | | Immunogen | Synthetic peptide|A synthetic peptide corresponding to a sequence within amino acids 1-55 of human TOMM7 (NP_061932.1). | | Source | Rabbit | | Category | Polyclonal Antibodies | | Application | WB, ELISA | | Cross Reactivity | Human | | SWISS | Q9P0U1 | | Protein Weight | 6kDa | | Shipping | Ice bag |

Biological Background: TOMM7 Function and Localization

• TOMM7 (Translocase of outer membrane 7 kDa subunit homolog, also known as TOM7) is a nucleus-encoded mitochondrial protein and a key subunit of the translocase of the outer membrane (TOM) complex.
• As a component of the TOM complex, TOMM7 is essential for the recognition and translocation of cytosolically synthesized mitochondrial preproteins, thereby maintaining mitochondrial protein homeostasis.
• At depolarized mitochondria, the TOM complex associates with the ion channel VDAC2 and PINK1 kinase; TOMM7 stabilizes PINK1 at the outer mitochondrial membrane, triggering PINK1-dependent mitophagy via recruitment of the E3 ubiquitin ligase PRKN (Parkin). Related references: PMID:40080546
• TOMM7 localizes exclusively to the mitochondrial outer membrane, consistent with its role in protein import.
• The protein is a small transmembrane protein with a predicted molecular weight of approximately 6 kDa (as confirmed by supplier data) and contains a single transmembrane helix.
• UniProt annotations highlight that TOMM7 is associated with disease variants, has a resolved 3D structure, and is part of the reference proteome set, underscoring its biomedical relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (residues 1-55) of human TOMM7; given the small protein size, consider using 15% SDS-PAGE or gradient gels and careful transfer optimization for detection by Western blot.
• For ELISA applications, peptide competition assays may help confirm binding specificity.
• As TOMM7 is an integral outer membrane protein, inclusion of mild detergents during sample preparation could improve protein solubilization and epitope exposure.
• Validation in the relevant sample system (e.g., human cell lines or tissue extracts) is advised to confirm detection of endogenous TOMM7.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibodies for mitochondrial research targets like TOMM7. Our supply capabilities encompass optimized polyclonal and monoclonal antibodies, antibody pairs, and ancillary reagents in bulk, along with rare-target raw-material sourcing to support your assay development from concept to clinic.