Anti-TOMM5 Rabbit Polyclonal Antibody for IF/ICC and ELISA - Q8N4H5

Core Product Specifications and Parameters

Biological Background: TOMM5 Function and Localization

• TOMM5 is a subunit of the translocase of the outer mitochondrial membrane (TOM) complex, which is essential for the recognition and import of cytosolically synthesized mitochondrial precursor proteins. Related references: PMID:40080546
• Upon mitochondrial depolarization, TOMM5 interacts with VDAC2 and PINK1 to stabilize PINK1 at the outer membrane, triggering Parkin-mediated mitophagy. Related references: PMID:40080546
• TOMM5 is an integral membrane protein located in the mitochondrial outer membrane, with a molecular weight of approximately 6 kDa.
• The protein undergoes acetylation and ubiquitination (isopeptide bond formation), as indicated by proteomics identification.
• Alternative splicing generates multiple transcript variants of TOMM5.
• Keywords from UniProt include 3D-structure, acetylation, alternative splicing, isopeptide bond, membrane, mitochondrion, mitochondrial outer membrane, protein transport, proteomics identification, reference proteome, transmembrane, transport, and Ubl conjugation.

Experimental Guidance and Technical Tips

• As the immunogen spans the N-terminal region (aa 1-51), the antibody may recognize full-length TOMM5 and any N-terminal-truncated isoforms. Validate specificity in your system using knockout or knockdown controls.
• For immunofluorescence (IF/ICC), consider optimizing fixation and permeabilization conditions to preserve mitochondrial membrane integrity.
• ELISA performance may be enhanced by using recombinant TOMM5 protein or peptide-coated plates as positive controls.
• Although western blotting is not listed, the small size of TOMM5 (~6 kDa) may require specialized transfer conditions if attempted; prefer the recommended applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with validated TOMM5 antibodies and complementary reagents for mitochondrial research. Our portfolio includes monoclonal/polyclonal antibodies, recombinant proteins, and ancillary materials for TOM complex and mitophagy pathway analysis. For custom or bulk sourcing needs, CamelBio provides expert consultation and streamlined procurement from concept to clinic.