Anti-TOMM40 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O96008

Core Product Specifications and Parameters

Biological Background: TOMM40 Function and Localization

• Protein Identity and Gene: TOMM40 (Translocase of Outer Mitochondrial Membrane 40) is also known as TOM40, Haymaker, PEREC1, and p38.5.
• TOM Complex Core Component: Functions as a channel-forming protein within the TOM complex, essential for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Related references: PMID:15644312, PMID:31206022, PMID:40080546
• Mitophagy Regulation: At depolarized mitochondria, associates with VDAC2 and PINK1 kinase, stabilizing PINK1 at the outer membrane and triggering downstream mitophagy via recruitment of E3 ubiquitin ligase PRKN. Related references: PMID:40080546
• Respiratory Chain Assembly: Plays a role in assembly of mitochondrial Complex I by forming a complex with BCAP31 and mediating translocation of Complex I components from the cytosol. Related references: PMID:31206022
• Subcellular Localization: Anchored in the mitochondrial outer membrane, serving as a porin and facilitating ion and protein transport.
• Alternative Splicing: The TOMM40 gene undergoes alternative splicing, generating multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–90) of human TOMM40; therefore, the antibody may recognize the N-terminal domain, which is exposed on the cytosolic side of the mitochondrial outer membrane.
• In Western blotting (WB), expect a band around 38 kDa, consistent with the full-length TOMM40 protein; multiple bands may appear due to alternative splicing.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), prioritize antigen retrieval and include a mitochondrial marker co-staining to confirm specific outer membrane localization.
• Cross-reactivity with mouse and rat TOMM40 has been demonstrated, enabling use in rodent tissue and cell models; however, validation in the specific experimental context is advised.
• For ELISA, consider the polyclonal nature may provide sensitivity for detecting denatured or native TOMM40; optimize coating and detection conditions as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutions with comprehensive access to IVD raw materials and support from concept to clinic. This anti-TOMM40 polyclonal antibody, targeting a key mitochondrial import receptor, is part of our extensive portfolio for mitochondrial research and clinical diagnostics. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your IVD development.