Anti-TOMM34 Rabbit Monoclonal Antibody - Q15785

Core Product Specifications and Parameters

Biological Background: TOMM34 Function and Localization

• TOMM34 is a mitochondrial import receptor subunit, also referred to as translocase of outer membrane 34 kDa subunit (TOM34), URCC3, or HTOM34P.
• It plays a critical role in importing cytosolically synthesized preproteins into mitochondria by binding the mature portion of precursor proteins and maintaining them in an unfolded, import-competent state.
• TOMM34 exhibits weak ATPase activity, which may support its chaperone-like function in keeping newly synthesized precursors soluble and competent for translocation.
• The protein localizes to the cytoplasm and the mitochondrial outer membrane, consistent with its role in early stages of protein import.
• TOMM34 is ubiquitously expressed across tissues, reflecting its general role in mitochondrial biogenesis.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, as identified by proteomic studies.
• Structural features include tetratricopeptide (TPR) repeats, which mediate protein-protein interactions.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–309 of human TOMM34 (full-length), suggesting the antibody may recognize both native and denatured epitopes; consider this when designing detection protocols.
• For Western blot (WB), expect TOMM34 to migrate at ~35 kDa; validate expression levels in your sample system, as expression is ubiquitous but may vary.
• For immunohistochemistry (IHC-P), test antibody performance on formalin-fixed paraffin-embedded human or rat tissues with appropriate antigen retrieval; optimization of dilution and detection may be necessary.
• For ELISA, the recombinant protein immunogen suggests potential compatibility with sandwich or indirect formats, but experimental titration is recommended to determine optimal working concentration.

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