Anti-TOMM22 Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q9NS69

Core Product Specifications and Parameters

Biological Background: TOMM22 Function and Localization

• TOMM22 serves as the central receptor component of the translocase of the outer membrane (TOM) complex, essential for recognition and import of cytosolically synthesized mitochondrial preproteins. Related references: PMID:40080546
• Together with the peripheral receptor TOMM20, it functions as the transit peptide receptor, facilitating preprotein movement into the translocation pore. Related references: PMID:10982837
• At depolarized mitochondria, the TOM complex interacts with VDAC2 and PINK1 kinase, stabilizing PINK1 on the outer membrane and triggering PRKN-mediated mitophagy. Related references: PMID:40080546
• Required for the translocation of cytochrome P450 monooxygenases across the mitochondrial outer membrane.
• Ubiquitously expressed across human tissues, reflecting its fundamental role in mitochondrial biogenesis.
• Localizes to the mitochondrial outer membrane and contains a single transmembrane helix.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity or protein interactions.
• Theoretical molecular weight is approximately 15.5 kDa, and it migrates at around 16 kDa in SDS‑PAGE.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N‑terminal region (aa 1–100) of human TOMM22; antibody specificity can be verified using knockout controls or overexpression experiments.
• For Western blot, a single band at approximately 16 kDa is expected; mitochondrial‑enriched samples may enhance signal clarity.
• In immunohistochemistry (paraffin), antigen retrieval using microwave‑heated citrate buffer (pH 6.0) is recommended to improve detection of the membrane‑associated protein.
• For immunoprecipitation, pre‑clearing of lysates and the use of low‑salt lysis buffers may help preserve native protein interactions.
• ELISA can be performed by coating plates with the immunizing peptide or recombinant TOMM22 protein to capture the antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD raw materials, technical services, and tailored consulting for mitochondrial research applications. In addition to this TOMM22 antibody, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development pipeline.