Anti-TOMM22 Polyclonal Antibody for WB and ELISA - Q9NS69

Core Product Specifications and Parameters

Biological Background: TOMM22 Function and Localization

• TOMM22 is the central receptor of the translocase of the outer membrane (TOM) complex, essential for mitochondrial preprotein import. Related references: PMID:40080546
• Together with TOMM20, it functions as a transit peptide receptor, facilitating preprotein movement into the translocation pore. Related references: PMID:10982837
• At depolarized mitochondria, TOMM22 associates with VDAC2 and PINK1, stabilizing PINK1 on the outer membrane to trigger mitophagy via PRKN recruitment. Related references: PMID:40080546
• The protein is a single-pass transmembrane protein located in the mitochondrial outer membrane.
• Post-translational modifications include acetylation and phosphorylation, regulating its function.
• Ubiquitously expressed across tissues, reflecting its fundamental role in mitochondrial biogenesis.
• It is also required for translocation of cytochrome P450 monooxygenases across the outer membrane.
• The gene has several aliases: 1C9-2, TOM22, MST065, and MSTP065.

Experimental Guidance and Technical Tips

• For Western blot, the expected protein weight is approximately 16 kDa; consider running a gradient gel for better resolution of small proteins.
• The immunogen corresponds to the N-terminal region (aa 1-83); validate specificity using siRNA knockdown or knockout cell lysates.
• For ELISA, use a standard sandwich format with a matched detection antibody if available.
• As a polyclonal antibody, lot-to-lot consistency should be verified in the intended application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing bridge for IVD raw materials, connecting diagnostic manufacturers and researchers with validated reagents. This anti-TOMM22 antibody supports mitochondrial biology and mitophagy research. Our capabilities span validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—streamlining your workflow from concept to clinic.