Anti-TOM70 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O94826

Core Product Specifications and Parameters

Biological Background: TOM70 Function and Localization

• TOM70 (Mitochondrial import receptor subunit TOM70), also known as TOMM70A or translocase of outer membrane 70 kDa subunit, is encoded by the TOMM70 gene (alternative names: KIAA0719, TOM70).
• Acts as a receptor of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex), mediating the import of mitochondrial precursor proteins.
  Related references:
  PMID:12526792
• Recognizes and translocates mitochondrial preproteins from the cytosol into the mitochondria in a chaperone-dependent manner, involving HSP70 and HSP90.
  Related references:
  PMID:12526792 | PMID:35025629
• Mediates TBK1 and IRF3 activation induced by MAVS upon Sendai virus infection, contributing to host antiviral innate immune responses.
  Related references:
  PMID:20628368 | PMID:25609812 | PMID:32728199
• During Sendai virus infection, recruits an HSP90AA1:IRF3:BAX complex to the mitochondrion, triggering apoptosis.
  Related references:
  PMID:25609812
• Localizes to the mitochondrial outer membrane, where it functions as a key component of the protein import machinery.
• Subject to various post-translational modifications including acetylation, phosphorylation, methylation, and ubiquitin-like conjugation, which may regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 245–608 of human TOM70, a region that excludes the N-terminal transmembrane domain; the antibody is expected to recognize the cytoplasmic domain.
• For Western blotting, the predicted molecular weight of TOM70 is ~67 kDa (UniProt: 67.5 kDa), but post-translational modifications may result in a band shift; validate using appropriate controls.
• For immunofluorescence/ICC, verify mitochondrial localization by co-staining with a mitochondrial marker to confirm specificity.
• For ELISA, consider using a recombinant TOM70 protein or peptide as coating antigen; optimize the dilution in your assay system.
• Since this is a polyclonal antibody, lot-to-lot consistency should be verified and the optimal working dilution determined empirically in the relevant sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes seamless access to a comprehensive portfolio of IVD raw materials. This anti-TOM70 antibody supports mitochondrial biology and antiviral signaling studies, and can be integrated into validated immunoassay workflows. From validated antibody pairs and bulk ancillary reagents to rare-target raw-material sourcing, our supply chain ensures that you receive consistent, high-quality materials tailored to your diagnostic development needs.