Anti-TOM20 Rat Monoclonal Antibody for WB, IF, IHC, ELISA - Q15388

Core Product Specifications and Parameters

Biological Background: TOM20 Function and Localization

• TOMM20 (also known as TOM20, MAS20, MOM19) is the human homolog of the mitochondrial import receptor subunit TOM20, a core component of the translocase of the outer membrane (TOM) complex essential for mitochondrial protein import. Related references: PMID:40080546
• Together with TOMM22, it acts as the transit peptide receptor at the mitochondrial surface, recognizing and guiding cytosolically synthesized preproteins into the TOM40 translocation pore. Related references: PMID:18331822
• TOMM20 associates with VDAC2 and PINK1 kinase at depolarized mitochondria, stabilizing PINK1 to trigger mitophagy via recruitment of the E3 ubiquitin ligase PRKN. Related references: PMID:40080546
• It is required for the translocation of cytochrome P450 monooxygenases across the mitochondrial outer membrane.
• TOMM20 localizes to the mitochondrial outer membrane and contains a single transmembrane helix.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin conjugation (Ubl).
• The protein has a molecular weight of approximately 16.3 kDa and is classified under reference proteome.

Experimental Guidance and Technical Tips

• Since the antibody is raised against recombinant human TOMM20, it may recognize the native protein in WB, IF, and IHC applications. For western blotting, expect a band near 16 kDa; consider using mitochondrial-enriched lysates to enhance signal.
• For immunofluorescence, anticipate mitochondrial outer membrane staining; co-staining with mitochondrial markers is suggested.
• When using for immunohistochemistry, optimize antigen retrieval conditions and validate with appropriate tissue controls.
• The antibody reacts with human, mouse, and rat samples; however, cross-reactivity in other species should be verified experimentally.
• For ELISA, use the antibody as capture or detection reagent and optimize coating conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner, providing diagnostic manufacturers and research labs with access to high-quality IVD raw materials like this anti-TOM20 monoclonal antibody, along with validated antibody pairs and ancillary reagents. Our catalog supports mitochondrial biology and disease research applications, and our team can assist in sourcing rare or custom targets to streamline your assay development.