Anti-TOM20 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q15388

Core Product Specifications and Parameters

Biological Background: TOM20 Function and Localization

• TOM20, also known as mitochondrial import receptor subunit TOM20 homolog, MAS20, or MOM19, is a central component of the translocase of the outer mitochondrial membrane (TOM) complex.
• Acts as a receptor for cytosolically synthesized mitochondrial preproteins, recognizing transit peptides together with TOMM22 and channeling them into the TOM40 pore for import (PubMed:18331822). Related references: PMID:18331822
• Plays a critical role in mitochondrial quality control by interacting with VDAC2 and PINK1 at depolarized mitochondria, stabilizing PINK1 to initiate mitophagy via PRKN recruitment (PubMed:40080546). Related references: PMID:40080546
• Required for the translocation of cytochrome P450 monooxygenases across the mitochondrial outer membrane.
• Localized exclusively to the mitochondrion outer membrane.
• Subject to post-translational modifications including phosphorylation and ubiquitination; features a single transmembrane helix.
• Contains key structural elements annotated as phosphoprotein, isopeptide bond, and transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the cytoplasmic region (amino acids 20-145) of human TOM20, which is highly conserved, likely accounting for the observed cross-reactivity with mouse and rat. Verify reactivity in your specific sample system if using other species.
• For Western blotting, expect a specific band around 16 kDa. Optimize loading and detection conditions for your samples.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), appropriate antigen retrieval and permeabilization may be necessary to access the mitochondrial outer membrane epitope.
• As a recombinant monoclonal antibody, this product offers consistent lot-to-lot performance, suitable for quantitative applications like ELISA and IP.
• Validate the antibody’s performance in your specific assay format and sample type before routine use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to premium IVD raw materials, supported by technical expertise from concept to clinic. This anti-TOM20 rabbit monoclonal antibody is part of our portfolio for mitochondrial biology and mitophagy research, complemented by validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Simplify your assay development with our integrated procurement platform.