Anti-TOM20 Monoclonal Antibody (Conjugated) for WB and IF - Q15388

Core Product Specifications and Parameters

Biological Background: TOM20 Function and Localization

• Mitochondrial import receptor subunit TOM20 homolog, also designated MAS20, MOM19, or TOM20, is encoded by the TOMM20 gene.
• Central receptor component of the translocase of the outer membrane (TOM) complex; together with TOMM22, it recognizes and binds cytosolic mitochondrial preproteins via their N-terminal targeting sequences, directing them into the TOM40 translocation pore. Related references: PMID:40080546 PMID:18331822
• At depolarized mitochondria, the TOM complex associates with VDAC2 and PINK1 kinase; this interaction stabilizes PINK1 on the outer membrane and triggers mitophagy through the recruitment of the E3 ubiquitin ligase PRKN (Parkin). Related references: PMID:40080546
• Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases (by similarity).
• Subcellular localization: firmly anchored in the mitochondrial outer membrane via a single C-terminal transmembrane helix (residues 126–145), with the N-terminal domain exposed to the cytosol.
• Post-translational modifications include phosphorylation at multiple residues and isopeptide bonds (Ubl conjugation); these may regulate receptor activity and protein turnover.
• A key player in mitochondrial biogenesis, quality control, and cell survival, making it a valuable target in studies of neurodegeneration, metabolic disorders, and cancer.

Experimental Guidance and Technical Tips

• This antibody is pre-labeled with , a far-red fluorescent dye, permitting direct detection without secondary antibodies in immunofluorescence (IF/ICC, IF-P) and in western blotting (WB) when using near-infrared imaging systems.
• The immunogen encompasses amino acids 20–145 of human TOM20, covering the cytosolic receptor domain; enriching mitochondrial fractions or using organelle-specific markers can improve signal specificity.
• Demonstrates cross-reactivity with human, mouse, and rat TOM20; validate in the appropriate cell line (e.g., HeLa, HepG2, NIH/3T3) or tissue.
• For WB, expect a band migrating at approximately 16 kDa; for IF, co-staining with a mitochondrial marker (e.g., anti-COX IV, MitoTracker) confirms outer membrane localization.
• Optimization of antibody dilution is recommended; typical starting concentrations for conjugated antibodies range from 1:50 to 1:200 for IF and 1:500 to 1:2000 for WB, but must be titrated in the experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, CROs, and academic labs a streamlined supply chain for IVD raw materials, including a curated selection of mitochondrial biology reagents. In addition to this -conjugated anti-TOM20 monoclonal antibody, we provide validated antibody pairs, recombinant proteins, and bulk ancillary reagents to accelerate your assay development. Contact our specialists for custom sourcing of rare targets or to discuss integrated technical support from concept to clinic.